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Open AccessArticle

Vitamin B Complex Treatment Attenuates Local Inflammation after Peripheral Nerve Injury

1
Institute of Physiology and Biochemistry “Ivan Djaja”, Faculty of Biology, University of Belgrade, 11000 Belgrade, Serbia
2
Institute for Orthopedic Surgery “Banjica”, 11000 Belgrade, Serbia
3
Institute for Medical Research, Faculty of Medicine, Military Medical Academy, University of Defense in Belgrade, 11000 Belgrade, Serbia
4
Department of Neurobiology, Institute for Biological Research “Sinisa Stankovic”, National Institute of Republic of Serbia, University of Belgrade, 11060 Belgrade, Serbia
*
Author to whom correspondence should be addressed.
These authors contributed equally to this study.
Academic Editor: Eduardo Sobarzo-Sánchez
Molecules 2019, 24(24), 4615; https://doi.org/10.3390/molecules24244615
Received: 18 October 2019 / Revised: 2 December 2019 / Accepted: 13 December 2019 / Published: 17 December 2019
(This article belongs to the Special Issue Natural Products for Neurodegenerative Diseases)
Peripheral nerve injury (PNI) leads to a series of cellular and molecular events necessary for axon regeneration and reinnervation of target tissues, among which inflammation is crucial for the orchestration of all these processes. Macrophage activation underlies the pathogenesis of PNI and is characterized by morphological/phenotype transformation from proinflammatory (M1) to an anti-inflammatory (M2) type with different functions in the inflammatory and reparative process. The aim of this study was to evaluate influence of the vitamin B (B1, B2, B3, B5, B6, and B12) complex on the process of neuroinflammation that is in part regulated by l-type CaV1.2 calcium channels. A controlled transection of the motor branch of the femoral peripheral nerve was used as an experimental model. Animals were sacrificed after 1, 3, 7, and 14 injections of vitamin B complex. Isolated nerves were used for immunofluorescence analysis. Treatment with vitamin B complex decreased expression of proinflammatory and increased expression of anti-inflammatory cytokines, thus contributing to the resolution of neuroinflammation. In parallel, B vitamins decreased the number of M1 macrophages that expressed the CaV1.2 channel, and increased the number of M2 macrophages that expressed this channel, suggesting their role in M1/M2 transition after PNI. In conclusion, B vitamins had the potential for treatment of neuroinflammation and neuroregeneration and thereby might be an effective therapy for PNI in humans. View Full-Text
Keywords: peripheral nerve injury; neuroinflammation; regeneration; M1/M2 macrophages; calcium channels; vitamin B complex therapy peripheral nerve injury; neuroinflammation; regeneration; M1/M2 macrophages; calcium channels; vitamin B complex therapy
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MDPI and ACS Style

Ehmedah, A.; Nedeljkovic, P.; Dacic, S.; Repac, J.; Draskovic Pavlovic, B.; Vucevic, D.; Pekovic, S.; Bozic Nedeljkovic, B. Vitamin B Complex Treatment Attenuates Local Inflammation after Peripheral Nerve Injury. Molecules 2019, 24, 4615.

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