Quantitative Analysis of Cold Stress Inducing Lipidomic Changes in Shewanella putrefaciens Using UHPLC-ESI-MS/MS
Abstract
:1. Introduction
2. Materials and Methods
2.1. Pretreatment of Samples
2.2. Lipids Separation by UHPLC
2.3. Lipids Quantification by Mass Spectrometric Analysis
2.4. External Calibration Method
2.5. Lipidomic Data Processing
3. Results and Discussion
3.1. Changes in Lipids Content
3.2. Changes in Content of Different Compositional Lipid Species
3.3. Changes in the Unsaturation Level of Compositional Lipid Species in Response to Cold Stress
4. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sample Availability: Samples of the compounds are not available from the authors. |
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Gao, X.; Liu, W.; Mei, J.; Xie, J. Quantitative Analysis of Cold Stress Inducing Lipidomic Changes in Shewanella putrefaciens Using UHPLC-ESI-MS/MS. Molecules 2019, 24, 4609. https://doi.org/10.3390/molecules24244609
Gao X, Liu W, Mei J, Xie J. Quantitative Analysis of Cold Stress Inducing Lipidomic Changes in Shewanella putrefaciens Using UHPLC-ESI-MS/MS. Molecules. 2019; 24(24):4609. https://doi.org/10.3390/molecules24244609
Chicago/Turabian StyleGao, Xin, Wenru Liu, Jun Mei, and Jing Xie. 2019. "Quantitative Analysis of Cold Stress Inducing Lipidomic Changes in Shewanella putrefaciens Using UHPLC-ESI-MS/MS" Molecules 24, no. 24: 4609. https://doi.org/10.3390/molecules24244609