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Open AccessArticle

The Biochemical and Functional Characterization of M28 Aminopeptidase Protein Secreted by Acanthamoeba spp. on Host Cell Interaction

by Jian-Ming Huang 1,†, Yao-Tsung Chang 2,† and Wei-Chen Lin 1,3,4,*
1
Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan
2
Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan
3
Department of Parasitology, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan
4
Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Aimin Liu
Molecules 2019, 24(24), 4573; https://doi.org/10.3390/molecules24244573
Received: 30 October 2019 / Revised: 11 December 2019 / Accepted: 12 December 2019 / Published: 13 December 2019
(This article belongs to the Special Issue Design and Application of Metal-Binding Proteins)
Acanthamoeba are a free-living protozoan whose pathogenic strain can cause severe human diseases, such as granulomatous encephalitis and keratitis. As such, the pathogenic mechanism between humans and Acanthamoeba is still unknown. In our previous study, we identified the secreted Acanthamoeba M28 aminopeptidase (M28AP) and then suggested that M28AP can degrade human C3b and iC3b for inhibiting the destruction of Acanthamoeba spp. with the human immune response. We constructed the produced the recombinant M28AP from a CHO cell, which is a mammalian expression system, to characterize the biochemical properties of Acanthamoeba M28AP. The recombinant M28AP more rapidly hydrolyzed Leu-AMC than Arg-AMC and could be inhibited by EDTA treatment. We show that recombinant M28AP can be delivered into the individual cell line and cause cell line apoptosis in a co-culture model. In conclusion, we successfully investigated the potential molecular characteristics of M28AP. View Full-Text
Keywords: Acanthamoeba spp.; secreted protein; M28AP; apoptosis Acanthamoeba spp.; secreted protein; M28AP; apoptosis
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MDPI and ACS Style

Huang, J.-M.; Chang, Y.-T.; Lin, W.-C. The Biochemical and Functional Characterization of M28 Aminopeptidase Protein Secreted by Acanthamoeba spp. on Host Cell Interaction. Molecules 2019, 24, 4573.

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