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Glucosinolates in Sisymbrium officinale (L.) Scop.: Comparative Analysis in Cultivated and Wild Plants and in Vitro Assays with T2Rs Bitter Taste Receptors

1
Department of Food, Environmental and Nutritional Sciences-DeFENS, University of Milan, Via Celoria 2, I-20133 Milano, Italy
2
Department of Agricultural and Environmental Sciences-DISAA, University of Milan, Via Celoria 2, I-20133 Milano, Italy
3
IMAX Discovery GmbH, Otto-Hahn-Straße, 15, 44227 Dortmund, Germany
4
Axxam S.p.A. Via Meucci, 3, 20091 Bresso, Italy
*
Author to whom correspondence should be addressed.
Molecules 2019, 24(24), 4572; https://doi.org/10.3390/molecules24244572
Received: 12 November 2019 / Revised: 6 December 2019 / Accepted: 9 December 2019 / Published: 13 December 2019
Sisymbrium officinale (L.) Scop., commonly known as “hedge mustard” or “the singer’s plant” is a wild plant common in Eurasian regions. Its cultivation is mainly dedicated to herboristic applications and it has only recently been introduced into Italy. The active botanicals in S. officinale are glucosinolates, generally estimated by using UV or high-performance liquid chromatography (HPLC). Using both techniques, we measured the total glucosinolates from S. officinale in different parts of the plant as roots, leaves, seeds, and flowers. A comparison was made for cultivated and wild samples, and for samples obtained with different pre-treatment and fresh, frozen, and dried storage conditions. Cultivated and wild plants have a comparable amount of total glucosinolates, while drying procedures can reduce the final glucosinolates content. The content in glucoputranjivin, which is the chemical marker for glucosinolates in S. officinale, has been determined using HPLC and a pure reference standard. Glucoputranjivin and two isothiocyanates from S. officinale have been submitted to in vitro assays with the platform of bitter taste receptors of the T2Rs family. The results show that glucoputranjivin is a selective agonist of receptor T2R16. View Full-Text
Keywords: Sisymbrium officinale; glucosinolates; glucoputranjivin; T2Rs bitter taste receptors Sisymbrium officinale; glucosinolates; glucoputranjivin; T2Rs bitter taste receptors
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Borgonovo, G.; Zimbaldi, N.; Guarise, M.; Bedussi, F.; Winnig, M.; Vennegeerts, T.; Bassoli, A. Glucosinolates in Sisymbrium officinale (L.) Scop.: Comparative Analysis in Cultivated and Wild Plants and in Vitro Assays with T2Rs Bitter Taste Receptors. Molecules 2019, 24, 4572.

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