Anti-Biofilm Effects of Synthetic Antimicrobial Peptides Against Drug-Resistant Pseudomonas aeruginosa and Staphylococcus aureus Planktonic Cells and Biofilm
Abstract
:1. Introduction
2. Results and Discussion
2.1. Growth Inhibition of Planktonic Cells by Peptides
2.2. Inhibitory Kinetics of Peptides on Bacterial Growth
2.3. Biofilm Formation Inhibition
2.4. Reductive Effects of Peptides on Preformed Biofilms
2.5. Effect of Peptides on Biofilm Components
2.6. Biofilm Reduction in the Presence of Peptides
2.7. In Vivo Anti-Biofilm Action of PS Peptides
3. Materials and Methods
3.1. Materials
3.2. Peptide Synthesis by Solid-Phase Method
3.3. Bacterial Strains and Growth Conditions
3.4. Biofilm Susceptibility Assay
3.4.1. Growth Inhibition in Planktonic Bacterial Cells
3.4.2. Inhibition of Biofilm Formation Assay
3.4.3. Reductive Assay in Preformed Biofilm
3.5. Growth Inhibition Kinetics
3.6. Reductive EPS Analyses
3.7. Scanning Electron Microscopy
3.8. In Vivo Study
3.9. Statistical Analysis
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Sample Availability: Not available. |
Bacteria | MIC (µM (µg/mL)) | |||||
---|---|---|---|---|---|---|
PS1-2 | PS1-5 | PS1-6 | Gentamicin | Oxacillin | Erythromycin | |
P. aeruginosa | ||||||
ATCC 15692 | 2 (3.67) | 2 (4) | 2 (3.37) | 1 (0.96) | 4 (3.21) | - |
CCARM 2073 | 2 (3.67) | 2 (4) | 2 (3.37) | 256 (244) | 512 (411) | - |
CCARM 2075 | 2 (3.67) | 2 (4) | 1 (1.68) | 256 (244) | 512 (411) | - |
DRPa 4007 | 4 (7.34) | 2 (4) | 2 (3.37) | 512 (489) | 256 (206) | - |
DRPa 3241 | 2 (3.67) | 2 (4) | 2 (3.37) | 512 (489) | 128 (103) | - |
S. aureus | ||||||
ATCC 25923 | 4 (7.34) | 32 (64) | 2 (3.37) | - | 8 (6.42) | 2 (1.47) |
CCARM 3125 | 4 (7.34) | 16 (32) | 2 (3.37) | - | 512 (411) | 256 (188) |
CCARM 3709 | 2 (3.67) | 16 (32) | 2 (3.37) | - | 32 (25.7) | 128 (93.9) |
DRSa 3399 | 4 (7.34) | 2 (4) | 2 (3.37) | - | 512 (411) | 256 (188) |
DRSa 3518 | 2 (3.67) | 2 (4) | 2 (3.37) | - | 256 (206) | 512 (376) |
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Park, S.-C.; Lee, M.-Y.; Kim, J.-Y.; Kim, H.; Jung, M.; Shin, M.-K.; Lee, W.-K.; Cheong, G.-W.; Lee, J.R.; Jang, M.-K. Anti-Biofilm Effects of Synthetic Antimicrobial Peptides Against Drug-Resistant Pseudomonas aeruginosa and Staphylococcus aureus Planktonic Cells and Biofilm. Molecules 2019, 24, 4560. https://doi.org/10.3390/molecules24244560
Park S-C, Lee M-Y, Kim J-Y, Kim H, Jung M, Shin M-K, Lee W-K, Cheong G-W, Lee JR, Jang M-K. Anti-Biofilm Effects of Synthetic Antimicrobial Peptides Against Drug-Resistant Pseudomonas aeruginosa and Staphylococcus aureus Planktonic Cells and Biofilm. Molecules. 2019; 24(24):4560. https://doi.org/10.3390/molecules24244560
Chicago/Turabian StylePark, Seong-Cheol, Min-Young Lee, Jin-Young Kim, Hyeonseok Kim, Myunghwan Jung, Min-Kyoung Shin, Woo-Kon Lee, Gang-Won Cheong, Jung Ro Lee, and Mi-Kyeong Jang. 2019. "Anti-Biofilm Effects of Synthetic Antimicrobial Peptides Against Drug-Resistant Pseudomonas aeruginosa and Staphylococcus aureus Planktonic Cells and Biofilm" Molecules 24, no. 24: 4560. https://doi.org/10.3390/molecules24244560