Next Article in Journal
Simultaneous Determination and Quantification of Triterpene Saponins from Camellia sinensis Seeds Using UPLC-PDA-QTOF-MS/MS
Previous Article in Journal
Effects of Olive and Pomegranate By-Products on Human Microbiota: A Study Using the SHIME® In Vitro Simulator
Open AccessArticle

Surface Display of Antigen Protein VP8* of Porcine Rotavirus on Bacillus Subtilis Spores Using CotB as a Fusion Partner

1
College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
2
Chengdu Vocational College of Agricultural Science and Technology, Chengdu 611100, China
3
Technology Centre of Chengdu Custom, Chengdu 611100, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Molecules 2019, 24(20), 3793; https://doi.org/10.3390/molecules24203793
Received: 8 September 2019 / Revised: 11 October 2019 / Accepted: 18 October 2019 / Published: 22 October 2019
(This article belongs to the Section Chemical Biology)
Porcine rotavirus is a major cause of acute viral gastroenteritis in suckling piglets, and vaccination is considered to be an effective measure to control these infections. The development of a live mucosal vaccine using Bacillus subtilis spores as an antigen delivery vehicle is a convenient and attractive vaccination strategy against porcine rotavirus. In this study, a shuttle vector was constructed for the spore surface display of the spike protein VP8* from porcine rotavirus (the genotype was G5P[7]). A successful display of the CotB-VP8* fusion protein on the spore surface was confirmed by Western blot and immunofluorescence microscopy analysis. The capacity for immune response generated after immunization with the recombinant strain was evaluated in a mouse model. The intestinal fecal IgA and serum IgG were detected by enzyme-linked-immunosorbent serologic assay (ELISA). Importantly, recombinant strain spores could elicit strong specific mucosal and humoral immune responses. These encouraging results suggest that recombinant B. subtilis BV could provide a strategy for a potential novel application approach to the development of a new and safe mucosal subunit vaccine against porcine rotavirus. View Full-Text
Keywords: porcine rotavirus; VP8* protein; Bacillus subtilis; spore surface display; oral immunization; immune responses porcine rotavirus; VP8* protein; Bacillus subtilis; spore surface display; oral immunization; immune responses
Show Figures

Figure 1

MDPI and ACS Style

Li, W.; Feng, J.; Li, J.; Li, J.; Wang, Z.; Khalique, A.; Yang, M.; Ni, X.; Zeng, D.; Zhang, D.; Jing, B.; Luo, Q.; Pan, K. Surface Display of Antigen Protein VP8* of Porcine Rotavirus on Bacillus Subtilis Spores Using CotB as a Fusion Partner. Molecules 2019, 24, 3793.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop