Xanthommatin is Behind the Antioxidant Activity of the Skin of Dosidicus gigas
Abstract
:1. Introduction
2. Results
2.1. Isolation of the Bioactive Pigments
2.2. Antioxidant Activity
2.3. Structure Elucidation
2.4. Electrospray Ionization–Mass Spectrometry
3. Discussion
4. Materials and Methods
4.1. Sample Preparation
4.2. Pigment Extraction
4.3. Fractioning by Open Column Chromatography
4.4. Thin-Layer Chromatography
4.5. Antioxidant Activity
4.6. Spectroscopic Methods
4.7. Electrospray Ionization–Mass Spectrometry
4.8. Statistical Analysis
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Fraction | ABTS2 (IC50 mg mL−1) | FRAP2 (IC50 mg mL−1) | DPPH2 (IC50 mg mL−1) |
---|---|---|---|
F1 | 2.12 ± 0.11a | 6.54 ± 0.06c | 6.49 ± 0.04e |
F2 | 2.77 ± 0.08c | 4.15 ± 0.11b | 4.67 ± 0.03c |
F3 | 2.07 ± 0.11a | 2.52 ± 0.20a | 3.56 ± 0.08b |
F4 | 10.2 ± 0.02e | 12.34 ± 0.33d | 8.69 ± 0.09e |
F5 | 6.02 ± 0.05d | 6.89 ± 0.07c | 9.52 ± 1.01e |
F6 | 2.25 ± 0.02b | 3.98 ± 0.10b | 5.34 ± 0.05d |
F7 | 2.08 ± 0.02a | 2.25 ± 0.09a | 2.60 ± 0.04a |
F8 | 5.78 ± 1.02d | 7.30 ± 0.03c | 3.44 ± 0.09b |
Mixture of solvents | Proportion |
---|---|
Ethyl acetate/Methanol | 60:40 |
Ethyl acetate/Methanol | 40:60 |
Ethyl acetate/Methanol | 20:80 |
Methanol | 100 |
Acetic acid/Water | 5:95 |
Acetic acid/Water | 10:90 |
Ammonium hydroxide/Water | 4:96 |
Ammonium hydroxide/Water | 8:92 |
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Share and Cite
Chan-Higuera, J.E.; Santacruz-Ortega, H.d.C.; Carbonell-Barrachina, Á.A.; Burgos-Hernández, A.; Robles-Sánchez, R.M.; Cruz-Ramírez, S.G.; Ezquerra-Brauer, J.M. Xanthommatin is Behind the Antioxidant Activity of the Skin of Dosidicus gigas. Molecules 2019, 24, 3420. https://doi.org/10.3390/molecules24193420
Chan-Higuera JE, Santacruz-Ortega HdC, Carbonell-Barrachina ÁA, Burgos-Hernández A, Robles-Sánchez RM, Cruz-Ramírez SG, Ezquerra-Brauer JM. Xanthommatin is Behind the Antioxidant Activity of the Skin of Dosidicus gigas. Molecules. 2019; 24(19):3420. https://doi.org/10.3390/molecules24193420
Chicago/Turabian StyleChan-Higuera, Jesús Enrique, Hisila del Carmen Santacruz-Ortega, Ángel A. Carbonell-Barrachina, Armando Burgos-Hernández, Rosario Maribel Robles-Sánchez, Susana Gabriela Cruz-Ramírez, and Josafat Marina Ezquerra-Brauer. 2019. "Xanthommatin is Behind the Antioxidant Activity of the Skin of Dosidicus gigas" Molecules 24, no. 19: 3420. https://doi.org/10.3390/molecules24193420