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Open AccessArticle

RNA-seq Based Transcriptome Analysis of the Anti-Obesity Effect of Green Tea Extract Using Zebrafish Obesity Models

Graduate School of Regional Innovation Studies, Mie University, Tsu, Mie 514-8507, Japan
Mie University Zebrafish Drug Screening Center, Tsu, Mie 514-8507, Japan
Department of Integrative Pharmacology, Mie University Graduate School of Medicine, Tsu, Mie 514-8507, Japan
Department of Bioinformatics, Mie University Life Science Research Centre, Tsu, Mie 514-8507, Japan
Rohto Pharmaceutical Co., Ltd., Osaka 544-8666, Japan
UMOU Science Lab, Mastusaka, Mie 515-0303, Japan
Author to whom correspondence should be addressed.
Molecules 2019, 24(18), 3256;
Received: 14 June 2019 / Revised: 5 September 2019 / Accepted: 5 September 2019 / Published: 6 September 2019
(This article belongs to the Special Issue EGCG, Green Tea and Polyphenols)
Green tea is a popular beverage that is rich in polyphenolic compounds such as catechins. Its major content, (-)-epigallocatechin-3-gallate, has been shown to have beneficial effects on several diseases including cancer, metabolic syndrome, cardiovascular diseases, and neurodegenerative diseases. The aim of this study was to assess the anti-obesity effects and the underlying molecular mechanisms of green tea extract (GTE) using zebrafish larva and adult obesity models. We administered 100 μg/mL GTE to zebrafish larvae and performed a short-term obesogenic test. GTE significantly decreased the visceral adipose tissue volume induced by a high-fat diet. Oral administration (250 µg/g body weight/day) of GTE to adult diet-induced obese zebrafish also significantly reduced their visceral adipose tissue volume, with a reduction of plasma triglyceride and total cholesterol levels. To investigate the molecular mechanism underlying the GTE effects, we conducted RNA sequencing using liver tissues of adult zebrafish and found that GTE may ameliorate the obese phenotypes via the activation of Wnt/β-catenin and adenosine monophosphate-activated protein kinase (AMPK) pathway signaling. In addition, the comparative transcriptome analysis revealed that zebrafish and mammals may share a common molecular response to GTE. Our findings suggest that daily consumption of green tea may be beneficial for the prevention and treatment of obesity. View Full-Text
Keywords: green tea extract; visceral adipose tissue; obesity; zebrafish; RNA-seq green tea extract; visceral adipose tissue; obesity; zebrafish; RNA-seq
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Zang, L.; Shimada, Y.; Nakayama, H.; Kim, Y.; Chu, D.-C.; Juneja, L.R.; Kuroyanagi, J.; Nishimura, N. RNA-seq Based Transcriptome Analysis of the Anti-Obesity Effect of Green Tea Extract Using Zebrafish Obesity Models. Molecules 2019, 24, 3256.

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