Next Article in Journal
Amphiphilic Block Copolymer PCL-PEG-PCL as Stationary Phase for Capillary Gas Chromatographic Separations
Next Article in Special Issue
Anti-Inflammatory Effect of Erinacine C on NO Production Through Down-Regulation of NF-κB and Activation of Nrf2-Mediated HO-1 in BV2 Microglial Cells Treated with LPS
Previous Article in Journal
Treatment with Docosahexaenoic Acid Improves Epidermal Keratinocyte Differentiation and Ameliorates Inflammation in Human Keratinocytes and Reconstructed Human Epidermis Models
Previous Article in Special Issue
Fangchinoline, a Bisbenzylisoquinoline Alkaloid can Modulate Cytokine-Impelled Apoptosis via the Dual Regulation of NF-κB and AP-1 Pathways
Open AccessArticle

Bioactive Constituents from the Roots of Eurycoma longifolia

by Jingya Ruan 1,†, Zheng Li 1,†, Ying Zhang 2, Yue Chen 2, Mengyang Liu 2, Lifeng Han 1, Yi Zhang 1,2,* and Tao Wang 1,2,*
1
Tianjin State Key Laboratory of Modern Chinese Medicine, 312 Anshanxi Road, Nankai District, Tianjin 300193, China
2
Tianjin Key Laboratory of TCM Chemistry and Analysis, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 312 Anshanxi Road, Nankai District, Tianjin 300193, China
*
Authors to whom correspondence should be addressed.
Theses authors contributed equally to this work.
Academic Editor: Maria da Graça Costa G. Miguel
Molecules 2019, 24(17), 3157; https://doi.org/10.3390/molecules24173157
Received: 12 August 2019 / Revised: 25 August 2019 / Accepted: 28 August 2019 / Published: 30 August 2019
(This article belongs to the Special Issue Anti-Inflammatory Activity of Natural Products)
Four new phenolic components, eurylophenolosides A (1) and B (2), eurylolignanosides A (3) and B (4), along with twelve known compounds were isolated from the roots of Eurycoma longifolia Jack. The structure of these components was elucidated by using various spectral techniques and chemical reactions. Among the known isolates, syringaldehyde (12), 3-chloro-4-hydroxybenzoic acid (13), 3-chloro-4-hydroxyl benzoic acid-4-O-β-d-glucopyranoside (14), and isotachioside (15) were isolated from the Eurycoma genus for the first time. Further, the NMR data of 14 was reported here firstly. Meanwhile, the nitric oxide (NO) inhibitory activities of all compounds were examined in lipopolysaccharide (LPS)-stimulated RAW264.7 cells at 40 μM. As results, piscidinol A (6), 24-epi-piscidinol A (7), bourjotinolone A (10), and scopoletin (16) were found to play important role in suppressing NO levels without cytotoxicity. Furthermore, the Western blot method was used to investigate the mechanism of compounds 6, 7, 10, and 16 by analysing the level of inflammation related proteins, such as inducible nitric oxide synthase (iNOS), interleukin-6 (IL-6), and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) in LPS-stimulated RAW264.7 cells. Consequently, compounds 6, 7, 10, and 16 were found to significantly inhibit LPS-induced protein expression of IL-6, NF-κB and iNOS in NF-κB signaling pathway. Moreover, it was found that the protein expression inhibitory effects of 6, 7, and 16 exhibited in a dose-dependent manner. The mechanism may be related to the inhibition of the iNOS expressions through suppressing the IL-6-induced NF-κB pathway. View Full-Text
Keywords: Eurycoma longifolia roots; eurylophenolosides; eurylolignanosides; piscidinol A; 24-epi-piscidinol A; bourjotinolone A; scopoletin; RAW 246.7 cell; anti-inflammatory activity Eurycoma longifolia roots; eurylophenolosides; eurylolignanosides; piscidinol A; 24-epi-piscidinol A; bourjotinolone A; scopoletin; RAW 246.7 cell; anti-inflammatory activity
Show Figures

Figure 1

MDPI and ACS Style

Ruan, J.; Li, Z.; Zhang, Y.; Chen, Y.; Liu, M.; Han, L.; Zhang, Y.; Wang, T. Bioactive Constituents from the Roots of Eurycoma longifolia. Molecules 2019, 24, 3157.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop