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Enzymatic Reactions in a Lab-on-Valve System: Cholesterol Evaluations

1
LAQV, REQUIMTE, Department of Chemical Sciences, Laboratory of Applied Chemistry, Faculty of Pharmacy, Porto University, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal
2
Instituto de Química, Departamento de Química Analítica, Campus Universitário de Ondina, Universidade Federal da Bahia, 40170-115 Salvador, Bahia, Brazil
3
INCT de Energia e Ambiente–Instituto de Química, Campus Universitário de Ondina, Universidade Federal da Bahia, 40170-115 Salvador, Bahia, Brazil
*
Authors to whom correspondence should be addressed.
Academic Editor: Pawel Koscielniak
Molecules 2019, 24(16), 2890; https://doi.org/10.3390/molecules24162890
Received: 19 July 2019 / Revised: 6 August 2019 / Accepted: 7 August 2019 / Published: 9 August 2019
(This article belongs to the Special Issue Modern Flow Analysis)
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PDF [967 KB, uploaded 9 August 2019]
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Abstract

The micro sequential injection analysis / lab-on-valve (µSIA-LOV) system is a miniaturized SIA system resulting from the implementation of a lab-on-valve (LOV) atop of the selection valve. It integrates the detection cell and the sample processing channels into the same device, promoting the reduction of reagent consumption and waste generation, the improvement of the versatility, and the reduction of the time of analysis. All of these characteristics are really relevant to the implementation of enzymatic reactions. Additionally, the evaluation of cholesterol in serum samples is widely relevant in clinical diagnosis, since higher values of cholesterol in human blood are actually an important risk factor for cardiovascular problems. An automatic methodology was developed based on the µSIA-LOV system in order to evaluate its advantages in the implementation of enzymatic reactions performed by cholesterol esterase, cholesterol oxidase and peroxidase. Considering these reactions, the developed methodology was also used for the evaluation of cholesterol in human serum samples, showing reliable and accurate results. The developed methodology presented detection and quantification limits of 1.36 and 4.53 mg dL−1 and a linear range up to 40 mg dL−1. This work confirmed that this µSIA-LOV system is a simple, rapid, versatile, and robust analytical tool for the automatic implementation of enzymatic reactions performed by cholesterol esterase, cholesterol oxidase, and peroxidase. It is also a useful alternative methodology for the routine determinations of cholesterol in real samples, even when compared with other automatic methodologies. View Full-Text
Keywords: cholesterol; serum samples; lab-on-valve; automation; enzymatic reaction cholesterol; serum samples; lab-on-valve; automation; enzymatic reaction
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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MDPI and ACS Style

S. Barbosa, J.; L.C. Passos, M.; A. Korn, M.G.; M.F.S. Saraiva, M.L. Enzymatic Reactions in a Lab-on-Valve System: Cholesterol Evaluations. Molecules 2019, 24, 2890.

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