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Alnus Sibirica Extracts Suppress the Expression of Inflammatory Cytokines Induced by Lipopolysaccharides, Tumor Necrosis Factor-α, and Interferon-γ in Human Dermal Fibroblasts

1
Department of Physiology, College of Medicine, Chung-Ang University, Seoul 06973, Korea
2
Laboratory of Pharmacognosy and Natural Product based Medicine, College of Pharmacy, Chung-Ang University, Seoul 06973, Korea
3
College of Pharmacy, Chung-Ang University, Seoul 06973, Korea
*
Author to whom correspondence should be addressed.
Molecules 2019, 24(16), 2883; https://doi.org/10.3390/molecules24162883
Received: 15 July 2019 / Revised: 6 August 2019 / Accepted: 6 August 2019 / Published: 8 August 2019
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Abstract

The effects of Alnus sibirica (AS) extracts on cytokine expression induced by inflammatory stimulants were examined in human dermal fibroblasts (HDFs) and RAW264.7 cells. The anti-oxidative effect and effect on cell viability of AS extracts were evaluated, and four extracts with the highest anti-oxidative effects were selected. HDFs and RAW264.7 cells were treated with inflammatory stimulants, and the expression of cytokines involved in acute (IL-6 and IL-10) and chronic (IL-18) inflammation, the initiation of the immune response (IL-33), and non-specific immune responses (IL-1β, IL-8, and TNF-α) were determined using a reverse-transcription polymerase chain reaction. LPS increased the expression of all the cytokines, except for IL-18; however, AS extracts, particularly AS2 and AS4, reduced this increase, and TNF-α treatment markedly increased the expression of cytokines related to non-specific immune responses. IFN-γ treatment induced no significant changes, except for increased IL-33 expression in HDFs. AS extracts inhibited the increase in the expression of IL-33 and other cytokines in HDFs. Thus, the exposure of HDFs and RAW264.7 cells to inflammatory stimulants increased the expression of cytokines related to all the inflammatory processes. HDFs are involved not only in simple tissue regeneration but also in inflammatory reactions in the skin. AS2 and AS4 may offer effective therapy for related conditions. View Full-Text
Keywords: skin inflammation; atopic-like dermatitis; inflammatory stimulators; cytokines; Alnus sibirica; anti-inflammatory skin inflammation; atopic-like dermatitis; inflammatory stimulators; cytokines; Alnus sibirica; anti-inflammatory
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Choi, J.; Moon, S.; Bae, H.; Kim, Y.-W.; Lee, D.; Kim, S.; Seo, Y.; Wang, H.S.; Choi, Y.W.; Lee, M.W.; Ko, J.-H.; Lim, I.; Bang, H. Alnus Sibirica Extracts Suppress the Expression of Inflammatory Cytokines Induced by Lipopolysaccharides, Tumor Necrosis Factor-α, and Interferon-γ in Human Dermal Fibroblasts. Molecules 2019, 24, 2883.

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