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Open AccessFeature PaperArticle

Long-Chain Polyisoprenoids Are Synthesized by AtCPT1 in Arabidopsis thaliana

Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5a, 02-106 Warsaw, Poland
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Molecules 2019, 24(15), 2789; https://doi.org/10.3390/molecules24152789
Received: 28 June 2019 / Revised: 26 July 2019 / Accepted: 28 July 2019 / Published: 31 July 2019
(This article belongs to the Special Issue Plant Isoprenoids)
Arabidopsis roots accumulate a complex mixture of dolichols composed of three families, (i.e., short-, medium- and long-chain dolichols), but until now none of the cis-prenyltransferases (CPTs) predicted in the Arabidopsis genome has been considered responsible for their synthesis. In this report, using homo- and heterologous (yeast and tobacco) models, we have characterized the AtCPT1 gene (At2g23410) which encodes a CPT responsible for the formation of long-chain dolichols, Dol-18 to -23, with Dol-21 dominating, in Arabidopsis. The content of these dolichols was significantly reduced in AtCPT1 T-DNA insertion mutant lines and highly increased in AtCPT1-overexpressing plants. Similar to the majority of eukaryotic CPTs, AtCPT1 is localized to the endoplasmic reticulum (ER). Functional complementation tests using yeast rer2Δ or srt1Δ mutants devoid of medium- or long-chain dolichols, respectively, confirmed that this enzyme synthesizes long-chain dolichols, although the dolichol chains thus formed are somewhat shorter than those synthesized in planta. Moreover, AtCPT1 acts as a homomeric CPT and does not need LEW1 for its activity. AtCPT1 is the first plant CPT producing long-chain polyisoprenoids that does not form a complex with the NgBR/NUS1 homologue. View Full-Text
Keywords: polyisoprenoids; polyprenols; dolichols; cis-prenyltransferase; protein N-glycosylation; Arabidopsis thaliana; T-DNA insertion mutant; rer2Δ and srt1Δ Saccharomyces cerevisiae mutant polyisoprenoids; polyprenols; dolichols; cis-prenyltransferase; protein N-glycosylation; Arabidopsis thaliana; T-DNA insertion mutant; rer2Δ and srt1Δ Saccharomyces cerevisiae mutant
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Surowiecki, P.; Onysk, A.; Manko, K.; Swiezewska, E.; Surmacz, L. Long-Chain Polyisoprenoids Are Synthesized by AtCPT1 in Arabidopsis thaliana. Molecules 2019, 24, 2789.

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