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Open AccessArticle

Styphnolobium japonicum (L.) Schott Fruits Increase Stress Resistance and Exert Antioxidant Properties in Caenorhabditis elegans and Mouse Models

1
Pharmaceutical Biology Department, Faculty of Pharmacy and Biotechnology, German University in Cairo, Cairo 11835, Egypt
2
Biology Department, Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Im Neuenheimer Feld 364, Heidelberg 69120, Germany
3
Departmento de Biotecnologia em Plantas Medicinais, Universidade de Ribeirão Preto, 14096-900 Ribeirão Preto, Brazil
4
Pharmacology and Toxicology department, Faculty of Pharmacy, Cairo University, Cairo 11562, Egypt
*
Author to whom correspondence should be addressed.
Molecules 2019, 24(14), 2633; https://doi.org/10.3390/molecules24142633
Received: 22 June 2019 / Revised: 15 July 2019 / Accepted: 17 July 2019 / Published: 19 July 2019
(This article belongs to the Special Issue Herbal Medicines–Unraveling Their Molecular Mechanism)
Styphnolobium japonicum (L.) Schott is a popular Asian tree widely used in traditional medicine. The current study explored the potential stress resistance and antioxidant activities of its fruits. Phytochemical profiling of the hydroalcoholic fruit extract was done via high performance liquid chromatography-photodiode array-electrospray ionization-mass/mass (HPLC-PDA-ESI-MS/MS). Twenty four phenolic constituents were tentatively identified in the extract. The Caenorhabditis elegans (C. elegans) nematode model in addition to trimethyltin (TMT)-induced neurotoxicity mouse model were used for in vivo evaluation of its antioxidant properties. The ability of the extract to enhance stress resistance was manifested through increasing survival rate by 44.7% and decreasing basal reactive oxygen species (ROS) levels by 72.3% in C. elegans. In addition, the extract increased the levels of the stress response enzyme superoxide dismutase-3 (Sod-3) by 55.5% and decreased the expression of heat shock protein-16.2 (Hsp-16.2) in nematodes, which had been challenged by juglone, by 21%. Using a mouse model, the extract significantly decreased the expression of the oxidative stress marker malondialdehyde (MDA). Furthermore, an elevation in the levels of the antioxidant marker glutathione (GSH), SOD and heme oxygenase-1 (HO-1) enzymes were observed. Our findings imply that Styphnolobium japonicum has the potential to be used in future studies focusing on diseases associated with oxidative stress. View Full-Text
Keywords: Styphnolobium japonicum; Caenorhabditis elegans; oxidative stress; antioxidant; HPLC-PDA-ESI-MS/MS Styphnolobium japonicum; Caenorhabditis elegans; oxidative stress; antioxidant; HPLC-PDA-ESI-MS/MS
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MDPI and ACS Style

Thabit, S.; Handoussa, H.; Roxo, M.; Cestari de Azevedo, B.; S.E. El Sayed, N.; Wink, M. Styphnolobium japonicum (L.) Schott Fruits Increase Stress Resistance and Exert Antioxidant Properties in Caenorhabditis elegans and Mouse Models. Molecules 2019, 24, 2633. https://doi.org/10.3390/molecules24142633

AMA Style

Thabit S, Handoussa H, Roxo M, Cestari de Azevedo B, S.E. El Sayed N, Wink M. Styphnolobium japonicum (L.) Schott Fruits Increase Stress Resistance and Exert Antioxidant Properties in Caenorhabditis elegans and Mouse Models. Molecules. 2019; 24(14):2633. https://doi.org/10.3390/molecules24142633

Chicago/Turabian Style

Thabit, Sara; Handoussa, Heba; Roxo, Mariana; Cestari de Azevedo, Bruna; S.E. El Sayed, Nesrine; Wink, Michael. 2019. "Styphnolobium japonicum (L.) Schott Fruits Increase Stress Resistance and Exert Antioxidant Properties in Caenorhabditis elegans and Mouse Models" Molecules 24, no. 14: 2633. https://doi.org/10.3390/molecules24142633

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