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Open AccessArticle

Comparison of Retention Behavior between Supercritical Fluid Chromatography and Normal-Phase High-Performance Liquid Chromatography with Various Stationary Phases

1
Nacalai Tesque, Inc., Ishibashi 617-0004 17, Kaide-cho, Muko-shi, Kyoto, Japan
2
Division of Metabolomics, Medical Institute of Bioregulation, Kyushu University, Maidashi 812-8582 3-1-1, Higashi-ku, Fukuoka, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Toshitaka Funazukuri
Molecules 2019, 24(13), 2425; https://doi.org/10.3390/molecules24132425
Received: 22 April 2019 / Revised: 28 June 2019 / Accepted: 1 July 2019 / Published: 2 July 2019
(This article belongs to the Special Issue Supercritical Fluids and Green Chemistry)
The retention behavior of a wide variety of stationary phases was compared in supercritical fluid chromatography (SFC) and normal-phase high-performance liquid chromatography (NP-HPLC). We also attempted to elucidate the retention behavior in SFC by investigating the selectivity of the different stationary phases. SFC separation conditions with polar stationary phases, such as silica gel (SL) and diol (Diol) phases, operate via adsorptions that include hydrophilic and ionic interactions similar to those in NP-HPLC. Moreover, non-polar stationary phases, such as pentabromophenyl (PBr), pyrenylethyl (PYE), and octadecyl (C18), could be used despite the non-polar mobile phase conditions, because the dispersion and π-π interactions were stronger in SFC than in HPLC. These results reflect the selectivity of the stationary phase and its retention factor, thus providing useful information for the selection of appropriate stationary phases for particular analytes. View Full-Text
Keywords: supercritical fluid chromatography; normal-phase high performance liquid chromatography; stationary phase; retention behavior; dispersion interaction supercritical fluid chromatography; normal-phase high performance liquid chromatography; stationary phase; retention behavior; dispersion interaction
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Hirose, T.; Keck, D.; Izumi, Y.; Bamba, T. Comparison of Retention Behavior between Supercritical Fluid Chromatography and Normal-Phase High-Performance Liquid Chromatography with Various Stationary Phases. Molecules 2019, 24, 2425.

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