Next Article in Journal
Metabolites of Medicarpin and Their Distributions in Rats
Next Article in Special Issue
Efficacy of ARV-1502, a Proline-Rich Antimicrobial Peptide, in a Murine Model of Bacteremia Caused by Multi-Drug Resistant (MDR) Acinetobacter baumannii
Previous Article in Journal
Cytotoxic Effects of Newly Synthesized Heterocyclic Candidates Containing Nicotinonitrile and Pyrazole Moieties on Hepatocellular and Cervical Carcinomas
Previous Article in Special Issue
Insulin Release Mechanism Modulated by Toxins Isolated from Animal Venoms: From Basic Research to Drug Development Prospects
Article

Functional Fragments of AIMP1-Derived Peptide (AdP) and Optimized Hydrosol for Their Topical Deposition by Box-Behnken Design

1
College of Pharmacy, Chungnam National University, Daejeon 34134, Korea
2
CureBio Research Center, Suwon 16229, Korea
3
Laboratory Animal Resource Center, Korea Research Institute of Bioscience and Biotechnology, Ochang 28116, Korea
4
College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 08826, Korea
*
Authors to whom correspondence should be addressed.
Academic Editors: Paula A. C. Gomes, Stefania Galdiero and Cátia Teixeira
Molecules 2019, 24(10), 1967; https://doi.org/10.3390/molecules24101967
Received: 16 April 2019 / Revised: 14 May 2019 / Accepted: 22 May 2019 / Published: 22 May 2019
(This article belongs to the Special Issue Bioactive Peptides—From Therapy to Nutrition)
Aminoacyl-tRNA synthetase complex-interacting multifunctional protein 1 (AIMP1)-derived peptide (AdP) has been developed as a cosmeceutical ingredient for skin anti-aging given its fibroblast-activating (FA) and melanocyte-inhibiting (MI) functions. However, a suitable strategy for the topical delivery of AdP was required due to its low-permeable properties. In this study, FA and MI domains of AdP (FA-AdP and MI-AdP, respectively) were determined by functional domain mapping, where the activities of several fragments of AdP on fibroblast and melanocyte were tested, and a hydrosol-based topical delivery system for these AdP fragments was prepared. The excipient composition of the hydrosol was optimized to maximize the viscosity and drying rate by using Box-Behnken design. The artificial skin deposition of FA-AdP-loaded hydrosol was evaluated using Keshary-Chien diffusion cells equipped with Strat-M membrane (STM). The quantification of the fluorescent dye-tagged FA-AdP in STM was carried out by near-infrared fluorescence imaging. The optimized hydrosol showed 127-fold higher peptide deposition in STM than free FA-AdP (p < 0.05). This work suggests that FA- and MI-AdP are active-domains for anti-wrinkle and whitening activities, respectively, and the hydrosol could be used as a promising cosmetic formulation for the delivery of AdPs to the skin. View Full-Text
Keywords: AIMP1-derived peptide (AdP); cosmeceutical peptide; Box-Behnken design; hydrosol; topical delivery of peptides AIMP1-derived peptide (AdP); cosmeceutical peptide; Box-Behnken design; hydrosol; topical delivery of peptides
Show Figures

Figure 1

MDPI and ACS Style

Lee, J.-J.; Han, Y.-M.; Kwon, T.-W.; Kim, D.H.; Lee, H.S.; Jung, W.J.; Kim, J.; Kang, S.; Kim, S.K.; Cho, C.-W.; Lee, K.-R.; Kim, D.-D.; Park, M.C.; Lee, J.-Y. Functional Fragments of AIMP1-Derived Peptide (AdP) and Optimized Hydrosol for Their Topical Deposition by Box-Behnken Design. Molecules 2019, 24, 1967. https://doi.org/10.3390/molecules24101967

AMA Style

Lee J-J, Han Y-M, Kwon T-W, Kim DH, Lee HS, Jung WJ, Kim J, Kang S, Kim SK, Cho C-W, Lee K-R, Kim D-D, Park MC, Lee J-Y. Functional Fragments of AIMP1-Derived Peptide (AdP) and Optimized Hydrosol for Their Topical Deposition by Box-Behnken Design. Molecules. 2019; 24(10):1967. https://doi.org/10.3390/molecules24101967

Chicago/Turabian Style

Lee, Jeong-Jun, Young-Min Han, Tae-Wan Kwon, Dong H. Kim, Han S. Lee, Woo J. Jung, Jina Kim, Sujin Kang, Sang K. Kim, Cheong-Weon Cho, Kyeong-Ryoon Lee, Dae-Duk Kim, Min C. Park, and Jae-Young Lee. 2019. "Functional Fragments of AIMP1-Derived Peptide (AdP) and Optimized Hydrosol for Their Topical Deposition by Box-Behnken Design" Molecules 24, no. 10: 1967. https://doi.org/10.3390/molecules24101967

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop