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Determination of Kanamycin by High Performance Liquid Chromatography

1
College of Life Science, Yangtze University, Jingzhou 434025, China
2
Research and Development Sharing Platform of Hubei Province for Freshwater Product Quality and Safety, Yangtze University, Jingzhou 434025, China
*
Author to whom correspondence should be addressed.
Academic Editors: Marcello Locatelli, Angela Tartaglia, Dora Melucci, Abuzar Kabir, Halil Ibrahim Ulusoy and Victoria Samanidou
Molecules 2019, 24(10), 1902; https://doi.org/10.3390/molecules24101902
Received: 1 April 2019 / Revised: 15 May 2019 / Accepted: 15 May 2019 / Published: 17 May 2019
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PDF [7631 KB, uploaded 17 May 2019]
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Abstract

Kanamycin is an aminoglycoside antibiotic widely used in treating animal diseases caused by Gram-negative and Gram-positive infections. Kanamycin has a relatively narrow therapeutic index, and can accumulate in the human body through the food chain. The abuse of kanamycin can have serious side-effects. Therefore, it was necessary to develop a sensitive and selective analysis method to detect kanamycin residue in food to ensure public health. There are many analytical methods to determine kanamycin concentration, among which high performance liquid chromatography (HPLC) is a common and practical tool. This paper presents a review of the application of HPLC analysis of kanamycin in different sample matrices. The different detectors coupled with HPLC, including Ultraviolet (UV)/Fluorescence, Evaporative Light Scattering Detector (ELSD)/Pulsed Electrochemical Detection (PED), and Mass Spectrometry, are discussed. Meanwhile, the strengths and weaknesses of each method are compared. The pre-treatment methods of food samples, including protein precipitation, liquid-liquid extraction (LLE), and solid-phase extraction (SPE) are also summarized in this paper. View Full-Text
Keywords: Kanamycin; HPLC; sample pre-treatment; different detectors; food contamination Kanamycin; HPLC; sample pre-treatment; different detectors; food contamination
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Zhang, X.; Wang, J.; Wu, Q.; Li, L.; Wang, Y.; Yang, H. Determination of Kanamycin by High Performance Liquid Chromatography. Molecules 2019, 24, 1902.

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