Next Article in Journal
Chemical Composition and Biological Activities of Oregano Essential Oil and Its Fractions Obtained by Vacuum Distillation
Next Article in Special Issue
Synthesis of Graphene Oxide Based Sponges and Their Study as Sorbents for Sample Preparation of Cow Milk Prior to HPLC Determination of Sulfonamides
Previous Article in Journal
Discovery of 2-(1-(3-(4-Chloroxyphenyl)-3-oxo- propyl)pyrrolidine-3-yl)-1H-benzo[d]imidazole-4-carboxamide: A Potent Poly(ADP-ribose) Polymerase (PARP) Inhibitor for Treatment of Cancer
Previous Article in Special Issue
Liquid Phase and Microwave-Assisted Extractions for Multicomponent Phenolic Pattern Determination of Five Romanian Galium Species Coupled with Bioassays
Article Menu
Issue 10 (May-2) cover image

Export Article

Open AccessReview

Determination of Kanamycin by High Performance Liquid Chromatography

College of Life Science, Yangtze University, Jingzhou 434025, China
Research and Development Sharing Platform of Hubei Province for Freshwater Product Quality and Safety, Yangtze University, Jingzhou 434025, China
Author to whom correspondence should be addressed.
Academic Editors: Marcello Locatelli, Angela Tartaglia, Dora Melucci, Abuzar Kabir, Halil Ibrahim Ulusoy and Victoria Samanidou
Molecules 2019, 24(10), 1902;
Received: 1 April 2019 / Revised: 15 May 2019 / Accepted: 15 May 2019 / Published: 17 May 2019
PDF [7631 KB, uploaded 17 May 2019]


Kanamycin is an aminoglycoside antibiotic widely used in treating animal diseases caused by Gram-negative and Gram-positive infections. Kanamycin has a relatively narrow therapeutic index, and can accumulate in the human body through the food chain. The abuse of kanamycin can have serious side-effects. Therefore, it was necessary to develop a sensitive and selective analysis method to detect kanamycin residue in food to ensure public health. There are many analytical methods to determine kanamycin concentration, among which high performance liquid chromatography (HPLC) is a common and practical tool. This paper presents a review of the application of HPLC analysis of kanamycin in different sample matrices. The different detectors coupled with HPLC, including Ultraviolet (UV)/Fluorescence, Evaporative Light Scattering Detector (ELSD)/Pulsed Electrochemical Detection (PED), and Mass Spectrometry, are discussed. Meanwhile, the strengths and weaknesses of each method are compared. The pre-treatment methods of food samples, including protein precipitation, liquid-liquid extraction (LLE), and solid-phase extraction (SPE) are also summarized in this paper. View Full-Text
Keywords: Kanamycin; HPLC; sample pre-treatment; different detectors; food contamination Kanamycin; HPLC; sample pre-treatment; different detectors; food contamination

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Zhang, X.; Wang, J.; Wu, Q.; Li, L.; Wang, Y.; Yang, H. Determination of Kanamycin by High Performance Liquid Chromatography. Molecules 2019, 24, 1902.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top