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Article

Determination of 4-Hexylresorcinol in Shrimp Samples by Solid Phase Extraction Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

1
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, Lihu Avenue 1800, Wuxi 214122, Jiangsu, China
2
School of Fishery, Zhejiang Ocean University, Haida Road 1, Zhoushan 316021, Zhejiang, China
*
Author to whom correspondence should be addressed.
Molecules 2018, 23(9), 2173; https://doi.org/10.3390/molecules23092173
Received: 27 July 2018 / Revised: 17 August 2018 / Accepted: 24 August 2018 / Published: 29 August 2018
A method for the rapid determination of 4-hexylresorcinol (4-HR) residue in shrimp by solid phase extraction (SPE) ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was established. 4-HR was extracted twice with methanol, and the extract was formulated into methanol-water solution (1:1). After being cleaned up and concentrated by a PRIME HLB solid phase extraction column, the sample was analyzed by UPLC-MS/MS and quantitatively determined by an external standard method. The separation was performed with a gradient system consisting of water and acetonitrile as the mobile phase. Monitoring was performed by electrospray ionization (ESI) in negative ion mode using multiple ion reaction monitoring (MRM). Good linearity was obtained in the concentration range of 1.0–100.0 μg/L, with correlation coefficients larger than 0.999. The limit of detection (LOD) was 0.25 μg/kg and the limit of quantification (LOQ) was 0.80 μg/kg. The average recoveries of 4-HR at spiked concentrations of 2.40, 6.40 and 16 μg/kg ranged from 81.35% to 94.68% with the relative standard deviations (n = 6) from 3.57% to 6.86%. The results showed that the method is simple, fast, sensitive, reliable, and reproducible; thus, it could be used as a rapid confirmation and quantitative analysis method of 4-HR residue in aquatic products. View Full-Text
Keywords: solid phase extraction (SPE); ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS); shrimp samples; 4-Hexylresorcinol (4-HR) solid phase extraction (SPE); ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS); shrimp samples; 4-Hexylresorcinol (4-HR)
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MDPI and ACS Style

Hao, Y.; Gao, X.; Xia, W. Determination of 4-Hexylresorcinol in Shrimp Samples by Solid Phase Extraction Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry. Molecules 2018, 23, 2173. https://doi.org/10.3390/molecules23092173

AMA Style

Hao Y, Gao X, Xia W. Determination of 4-Hexylresorcinol in Shrimp Samples by Solid Phase Extraction Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry. Molecules. 2018; 23(9):2173. https://doi.org/10.3390/molecules23092173

Chicago/Turabian Style

Hao, Yunbin, Xuehui Gao, and Wenshui Xia. 2018. "Determination of 4-Hexylresorcinol in Shrimp Samples by Solid Phase Extraction Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry" Molecules 23, no. 9: 2173. https://doi.org/10.3390/molecules23092173

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