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Open AccessArticle

Deoxyschizandrin Loaded Liposomes on the Suppression Lipid Accumulation in 3T3-L1 Adipocytes

1
School of Integrated Traditional Chinese and Western Medicine, Binzhou Medical University, Yantai 264003, China
2
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China
*
Authors to whom correspondence should be addressed.
Molecules 2018, 23(9), 2158; https://doi.org/10.3390/molecules23092158
Received: 29 July 2018 / Revised: 20 August 2018 / Accepted: 22 August 2018 / Published: 27 August 2018
Deoxyschizandrin (DS) is a bioactive benzocyclooctadiene lignan found in the fruit of Schisandra chinensis. However, poor bioavailability and non-specificity of DS frequently caused low therapeutic efficacy. In the present study, DS-liposome (DS-lipo) was implemented to enhance the hepatic targeting and inhibition effects on adipocyte differentiation in 3T3-L1 cells. The formulations enabled encapsulation of as much as 24.14% DS. The DS-lipo prepared was about 73.08 nm, as measured by laser light scattering (LLS) morphology. In the visual field of a scanning electron microscope (SEM), the liposomes were spherical with similar size and uniform dispersion. Fluorescence live imaging study exhibited hepatic targeting of liposomes in vivo. Furthermore, High-Content Analysis (HCS) imaging microassay analyses revealed DS-lipo and DS reduced cytoplasmic lipid droplet in 3T3-L1 adipocytes, with the IC50 value of 8.68 μM and 31.08 μM, respectively. The lipid droplet accumulation inhibition rate of 10 μM DS-lipo was above 90%, which was even superior to the effect of 30 μM DS solution. The current findings suggest that DS-lipo was a therapeutic strategy for alleviating lipid-associated diseases and nonalcoholic fatty liver disease (NAFLD). View Full-Text
Keywords: deoxyschizandrin; nonalcoholic fatty liver disease; liposomes; lipid droplet; 3T3-L1 adipocyte deoxyschizandrin; nonalcoholic fatty liver disease; liposomes; lipid droplet; 3T3-L1 adipocyte
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Liu, X.; Wang, S.; Wu, Z.; Wang, Z.; Zheng, Q.; Li, D. Deoxyschizandrin Loaded Liposomes on the Suppression Lipid Accumulation in 3T3-L1 Adipocytes. Molecules 2018, 23, 2158.

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