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Molecules 2018, 23(9), 2152; https://doi.org/10.3390/molecules23092152

Development of a NIR Method for the In-Line Quantification of the Total Polyphenolic Content: A Study Applied on Ajuga genevensis L. Dry Extract Obtained in a Fluid Bed Process

1
Department of Medical Devices, Iuliu Hatieganu University of Medicine and Pharmacy, 4 Louis Pasteur Street, Cluj-Napoca 400439, Romania
2
Department of Pharmaceutical Botany, Iuliu Hatieganu University of Medicine and Pharmacy, 23 Gheorghe Marinescu Street, Cluj-Napoca 400337, Romania
3
Department of Pharmacognosy, Iuliu Hatieganu University of Medicine and Pharmacy, 12 Ion Creanga Street, Cluj-Napoca 400010, Romania
4
Department of Pharmaceutical Technology and Biopharmacy, Iuliu Hatieganu University of Medicine and Pharmacy, 41 Victor Babes Street, Cluj-Napoca 400012, Romania
Joint last co-authorship.
*
Author to whom correspondence should be addressed.
Received: 21 July 2018 / Revised: 7 August 2018 / Accepted: 24 August 2018 / Published: 27 August 2018
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Abstract

This study describes an innovative in-line near-infrared (NIR) process monitoring method for the quantification of the total polyphenolic content (TPC) of Ajuga genevensis dry extracts. The dry extract was obtained in a fluidized bed processor, by spraying and adsorbing a liquid extract onto an inert powder support. NIR spectra were recorded continuously during the extract’s spraying process. For the calibration of the in-line TPC quantification method, samples were collected during the entire process. The TPC of each sample was assessed spectroscopically, by applying a UV-Vis reference method. The obtained values were further used in order to develop a quality OPLS prediction model by correlating them with the corresponding NIR spectra. The final dry extract registered good flowability and compressibility properties, a concentration in active principles three times higher than the one of the liquid extract and an overall process yield of 85%. The average TPC’s recovery of the NIR in-line prediction method, compared with the reference UV-Vis one, was 98.7%, indicating a reliable monitoring method which provided accurate predictions of the TPC during the process, permitting a good process overview and enabling us to establish the process’s end point at the exact moment when the product reaches the desired TPC concentration. View Full-Text
Keywords: Ajuga genevensis; near-infrared spectroscopy; dry extract; fluid bed process; microNIR; in-line monitoring; total polyphenolic content Ajuga genevensis; near-infrared spectroscopy; dry extract; fluid bed process; microNIR; in-line monitoring; total polyphenolic content
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Gavan, A.; Colobatiu, L.; Mocan, A.; Toiu, A.; Tomuta, I. Development of a NIR Method for the In-Line Quantification of the Total Polyphenolic Content: A Study Applied on Ajuga genevensis L. Dry Extract Obtained in a Fluid Bed Process. Molecules 2018, 23, 2152.

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