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Molecules 2018, 23(9), 2090; https://doi.org/10.3390/molecules23092090

Study on the Quality Evaluation of Compound Danshen Preparations Based on the xCELLigence Real-Time Cell-Based Assay and Pharmacodynamic Authentication

1
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China
2
Department of Pathobiological Science, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA
3
Department of Chemical and Biomolecular Engineering, the Ohio State University, Columbus, OH 43210, USA
*
Author to whom correspondence should be addressed.
Received: 16 June 2018 / Revised: 18 August 2018 / Accepted: 19 August 2018 / Published: 21 August 2018
(This article belongs to the Special Issue Application of Novel Method in Pharmaceutical Study)
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Abstract

Objective: To perform a preliminary study on the quality evaluation of compound Danshen preparations based on the xCELLigence Real-Time Cell-based Assay (RTCA) system and make a pharmacodynamics verification. Methods: The compound Danshen was discussed as a methodological example, and the bioactivity of the compound Danshen preparations were evaluated by real-time cell electronic analysis technology. Meanwhile, an in vivo experiment on an acute blood stasis rat model was performed in order to verify this novel evaluation through the curative effect of dissipating blood stasis. Results: We determined the cell index (CI) and IC50 of the compound Danshen preparations and produced time/dose-dependent cell response profiles (TCRPs). The quality of the three kinds of compound Danshen preparations was evaluated through the RTCA data. The trend of CI and TCRPs reflected the effect of drugs on the cell (promoting or inhibiting), and it was verified that the results correlated with the biological activity of the drugs using a pharmacodynamics experiment. Conclusion: The RTCA system can be used to evaluate the quality of compound Danshen Preparations, and it can provide a new idea and new method for quantitatively characterizing the biological activity of traditional Chinese medicines (TCMs). View Full-Text
Keywords: compound Danshen preparations; xCELLigence; RTCA (Real-Time Cell-based Assay); quality evaluation compound Danshen preparations; xCELLigence; RTCA (Real-Time Cell-based Assay); quality evaluation
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Yan, G.; Zhu, Z.; Jin, L.; Chen, J.; Xie, H.; Miozzi, J.; Lei, F.; Wei, X.; Pan, J. Study on the Quality Evaluation of Compound Danshen Preparations Based on the xCELLigence Real-Time Cell-Based Assay and Pharmacodynamic Authentication. Molecules 2018, 23, 2090.

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