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Volume 23
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10.3390/molecules23082075
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Article

Antimicrobial Photodynamic Therapy Mediated by Curcumin-Loaded Polymeric Nanoparticles in a Murine Model of Oral Candidiasis

by
Vinicius Tatsuyuji Sakima
1,
Paula Aboud Barbugli
1,
Paulo Sérgio Cerri
1,
Marlus Chorilli
2,
Juliana Cabrini Carmello
1,
Ana Cláudia Pavarina
1 and
Ewerton Garcia de Oliveira Mima
1,*
1
School of Dentistry, São Paulo State University (UNESP), Araraquara 14801-903, São Paulo, Brazil
2
School of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara 14800-903, São Paulo, Brazil
*
Author to whom correspondence should be addressed.
Molecules 2018, 23(8), 2075; https://doi.org/10.3390/molecules23082075
Submission received: 14 July 2018 / Revised: 14 August 2018 / Accepted: 14 August 2018 / Published: 19 August 2018
(This article belongs to the Special Issue Nanomaterials for Phototherapeutic Applications)
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Abstract

Antimicrobial photodynamic therapy (aPDT) has been proposed as an alternative method for oral candidiasis (OC), while nanocarriers have been used to improve the water solubility of curcumin (CUR). The aim of this study is to encapsulate CUR in polymeric nanoparticles (NPs) and to evaluate its photodynamic effects on a murine model of OC. Anionic and cationic CUR-NP is synthesized using poly-lactic acid and dextran sulfate and then characterized. Female mice are immunosuppressed and inoculated with Candida albicans (Ca) to induce OC. aPDT is performed by applying CUR-NP or free CUR on the dorsum of the tongue, followed by blue light irradiation for five consecutive days. Nystatin is used as positive control. Afterward, Ca are recovered and cultivated. Animals are euthanized for histological, immunohistochemical, and DNA damage evaluation. Encapsulation in NP improves the water solubility of CUR. Nystatin shows the highest reduction of Ca, followed by aPDT mediated by free CUR, which results in immunolabelling of cytokeratins closer to those observed for healthy animals. Anionic CUR-NP does not show antifungal effect, and cationic CUR-NP reduces Ca even in the absence of light. DNA damage is associated with Ca infection. Consecutive aPDT application is a safe treatment for OC.
Keywords: photochemotherapy; nanoparticles; keratins; mice; Candida albicans; oral candidiasis; curcumin photochemotherapy; nanoparticles; keratins; mice; Candida albicans; oral candidiasis; curcumin

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MDPI and ACS Style

Sakima, V.T.; Barbugli, P.A.; Cerri, P.S.; Chorilli, M.; Carmello, J.C.; Pavarina, A.C.; Mima, E.G.d.O. Antimicrobial Photodynamic Therapy Mediated by Curcumin-Loaded Polymeric Nanoparticles in a Murine Model of Oral Candidiasis. Molecules 2018, 23, 2075. https://doi.org/10.3390/molecules23082075

AMA Style

Sakima VT, Barbugli PA, Cerri PS, Chorilli M, Carmello JC, Pavarina AC, Mima EGdO. Antimicrobial Photodynamic Therapy Mediated by Curcumin-Loaded Polymeric Nanoparticles in a Murine Model of Oral Candidiasis. Molecules. 2018; 23(8):2075. https://doi.org/10.3390/molecules23082075

Chicago/Turabian Style

Sakima, Vinicius Tatsuyuji, Paula Aboud Barbugli, Paulo Sérgio Cerri, Marlus Chorilli, Juliana Cabrini Carmello, Ana Cláudia Pavarina, and Ewerton Garcia de Oliveira Mima. 2018. "Antimicrobial Photodynamic Therapy Mediated by Curcumin-Loaded Polymeric Nanoparticles in a Murine Model of Oral Candidiasis" Molecules 23, no. 8: 2075. https://doi.org/10.3390/molecules23082075

APA Style

Sakima, V. T., Barbugli, P. A., Cerri, P. S., Chorilli, M., Carmello, J. C., Pavarina, A. C., & Mima, E. G. d. O. (2018). Antimicrobial Photodynamic Therapy Mediated by Curcumin-Loaded Polymeric Nanoparticles in a Murine Model of Oral Candidiasis. Molecules, 23(8), 2075. https://doi.org/10.3390/molecules23082075

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