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Molecules 2018, 23(8), 1953; https://doi.org/10.3390/molecules23081953

A Simple, Sensitive, and Reliable Method for the Simultaneous Determination of Multiple Antibiotics in Vegetables through SPE-HPLC-MS/MS

1
Key Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China
2
China-New Zealand Joint Laboratory for Soil Molecular Ecology, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China
3
Beijing Key Laboratory of Detection and Control of Spoilage microorganisms and Pesticide Residues in Agricultural Products, Beijing University of Agriculture, Beijing 102206, China
4
Scion, Private Bag 29237, Christchurch 8440, New Zealand
*
Author to whom correspondence should be addressed.
Received: 9 June 2018 / Revised: 25 June 2018 / Accepted: 28 June 2018 / Published: 6 August 2018
(This article belongs to the Section Analytical Chemistry)
Full-Text   |   PDF [2145 KB, uploaded 6 August 2018]   |  

Abstract

Antibiotics, widely used in livestock breeding, enter the environment through animal manure because of incomplete absorption in animals, especially the farmland ecosystem. Therefore, antibiotics may be adsorbed by plants and even become hazardous to human health through the food chain. In this study, a simple, sensitive, and reliable method was developed for the simultaneous determination of eleven antibiotics, including four sulfonamides, two tetracyclines, three fluoroquinolones, tylosin, and chloramphenicol in different vegetable samples using SPE-HPLC-MS/MS. Vegetable samples were extracted by acetonitrile added with hydrochloric acid (125:4, v/v). The extracts were enriched by circumrotating evaporation, and then cleaned through SPE on a hydrophilic-lipophilic balance (HLB) cartridge. All compounds were determined on a C18 reverse phase column through HPLC-MS/MS. The mean recoveries of 11 antibiotics from spiked samples of vegetables ranged from 71.4% to 104.0%. The limits of detection and quantification were 0.06–1.88 μg/kg and 0.20–6.25 μg/kg, respectively. The applicability of this technique demonstrated its good selectivity, high efficiency, and convenience by the analysis of 35 vegetable samples available from a vegetable greenhouse. Antibiotic residues in vegetables have aroused wide concern from the public. Therefore, standards should be established for antibiotic residues in vegetables to ensure food safety and human health. View Full-Text
Keywords: antibiotics; vegetable; SPE; HPLC-MS/MS antibiotics; vegetable; SPE; HPLC-MS/MS
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Feng, Y.; Zhang, W.-J.; Liu, Y.-W.; Xue, J.-M.; Zhang, S.-Q.; Li, Z.-J. A Simple, Sensitive, and Reliable Method for the Simultaneous Determination of Multiple Antibiotics in Vegetables through SPE-HPLC-MS/MS. Molecules 2018, 23, 1953.

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