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Molecules
Volume 23
Issue 8
10.3390/molecules23081912
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Article

Physicochemical Characterization, Antioxidant and Immunostimulatory Activities of Sulfated Polysaccharides Extracted from Ascophyllum nodosum

by
Ligen Chen
1,2,3,
Yan Wang
1,
Hui Yang
2,
Han Li
1,2,3,
Wei Xu
2,3,*,
Guijie Chen
4 and
Hongjun Zhu
1,*
1
College of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing 211816, China
2
School of Marine and Bioengineering, Yancheng Institute of Technology, Yancheng 224051, China
3
Jiangsu Key Laboratory of Biochemistry and Biotechnology of Marine Wetland, Yancheng Institute of Technology, Yancheng 224051, China
4
College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
*
Authors to whom correspondence should be addressed.
Molecules 2018, 23(8), 1912; https://doi.org/10.3390/molecules23081912
Received: 5 July 2018 / Revised: 24 July 2018 / Accepted: 26 July 2018 / Published: 31 July 2018
(This article belongs to the Collection Bioactive Compounds)
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Abstract

Polysaccharides from Ascophyllum nodosum (AnPS) were extracted and purified via an optimized protocol. The optimal extraction conditions were as follows: extraction time of 4.3 h, extraction temperature of 84 °C and ratio (v/w, mL/g) of extraction solvent (water) to raw material of 27. The resulting yield was 9.15 ± 0.23% of crude AnPS. Two fractions, named AnP1-1 and AnP2-1 with molecular weights of 165.92 KDa and 370.68 KDa, were separated from the crude AnPS by chromatography in DEAE Sepharose Fast Flow and Sephacryl S-300, respectively. AnP1-1 was composed of mannose, ribose, glucuronic acid, glucose and fucose, and AnP2-1 was composed of mannose, glucuronic acid, galactose and fucose. AnPS, AnP1-1 and AnP2-1 exhibited high scavenging activities against ABTS radical and superoxide radical, and showed protective effect on H2O2-induced oxidative injury in RAW264.7 cells. Furthermore, the immunostimulatory activities of AnP1-1 and AnP2-1 were evaluated by Caco-2 cells, the results showed both AnP1-1 and AnP2-1 could significantly promote the production of immune reactive molecules such as interleukin (IL)-8, IL-1β, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α. Therefore, the results suggest that AnPS and its two fractions may be explored as a potential functional food supplement. View Full-Text
Keywords: Ascophyllum nodosum; polysaccharides; antioxidant activity; immunostimulatory activity Ascophyllum nodosum; polysaccharides; antioxidant activity; immunostimulatory activity
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MDPI and ACS Style

Chen, L.; Wang, Y.; Yang, H.; Li, H.; Xu, W.; Chen, G.; Zhu, H. Physicochemical Characterization, Antioxidant and Immunostimulatory Activities of Sulfated Polysaccharides Extracted from Ascophyllum nodosum. Molecules 2018, 23, 1912. https://doi.org/10.3390/molecules23081912

AMA Style

Chen L, Wang Y, Yang H, Li H, Xu W, Chen G, Zhu H. Physicochemical Characterization, Antioxidant and Immunostimulatory Activities of Sulfated Polysaccharides Extracted from Ascophyllum nodosum. Molecules. 2018; 23(8):1912. https://doi.org/10.3390/molecules23081912

Chicago/Turabian Style

Chen, Ligen, Yan Wang, Hui Yang, Han Li, Wei Xu, Guijie Chen, and Hongjun Zhu. 2018. "Physicochemical Characterization, Antioxidant and Immunostimulatory Activities of Sulfated Polysaccharides Extracted from Ascophyllum nodosum" Molecules 23, no. 8: 1912. https://doi.org/10.3390/molecules23081912

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