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Open AccessArticle

Isolation and Antimicrobial Activity of Coumarin Derivatives from Fruits of Peucedanum luxurians Tamamsch

1
Department of Pharmacognosy with Medicinal Plant Unit, Medical University of Lublin, Chodzki, 120-093 Lublin, Poland
2
Department of Pharmacognosy, “Grigore T. Popa” University of Medicine and Pharmacy, 16 Universitatii Street, 700115 Iasi, Romania
3
Department of Pharmacognosy and Chemistry of Natural Products, School of Pharmacy, University of Athens, Zografou, 15771 Athens, Greece
4
School of Pharmaceutical Sciences, EPGL, University of Geneva, University of Lausanne, CMU, 1, Rue Michel Servet, 1211 Geneva 4, Switzerland
*
Author to whom correspondence should be addressed.
Molecules 2018, 23(5), 1222; https://doi.org/10.3390/molecules23051222
Received: 30 April 2018 / Revised: 14 May 2018 / Accepted: 16 May 2018 / Published: 20 May 2018
(This article belongs to the Section Natural Products Chemistry)
As a continuation of searching for phytoconstituents that act as promising agents for antimicrobial therapy, rare coumarins were isolated from fruits of Peucedanum luxurians and tested. In a first step, the content of major compounds in the aerial parts and fruits of P. luxurians were compared. The results clearly showed that the fruits with dichloromethane as a solvent yielded, in most cases, higher concentrations of almost all the analyzed coumarins than the aerial parts, with peucedanin detected as the most abundant compound with a concentration of 4563.94 ± 3.35 mg/100 g. Under this perspective, the dichloromethane extract from the fruits of P. luxurians was further submitted to high performance countercurrent chromatography with a mixture of n-hexane, ethyl acetate, methanol, and water 6:5:6:5 (v/v). Combination of HPCCC and prep-HPLC yielded 6′,7′-dihydroxybergamottin (1), officinalin (2), stenocarpin isobutyrate (3), officinalin isobutyrate (4), 8-methoxypeucedanin (5), and peucedanin (6). Isolated compounds were tested against several Gram-positive and Gram-negative bacteria strains. 6′,7′-Dihydroxybergamottin, peucedanin, and officinalin isobutyrate appeared to be the most active against all tested bacteria strains with minimum inhibitory concentration (MIC) values between 1.20 and 4.80 mg/mL. To the best of our knowledge, this is the first report about countercurrent isolation of mentioned coumarins, as well as the first information about their antimicrobial activity. View Full-Text
Keywords: Peucedanum luxurians Tamamsch.; Apiaceae; accelerated solvent extraction; purification; natural products; countercurrent chromatography Peucedanum luxurians Tamamsch.; Apiaceae; accelerated solvent extraction; purification; natural products; countercurrent chromatography
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MDPI and ACS Style

Widelski, J.; Luca, S.V.; Skiba, A.; Chinou, I.; Marcourt, L.; Wolfender, J.-L.; Skalicka-Wozniak, K. Isolation and Antimicrobial Activity of Coumarin Derivatives from Fruits of Peucedanum luxurians Tamamsch. Molecules 2018, 23, 1222. https://doi.org/10.3390/molecules23051222

AMA Style

Widelski J, Luca SV, Skiba A, Chinou I, Marcourt L, Wolfender J-L, Skalicka-Wozniak K. Isolation and Antimicrobial Activity of Coumarin Derivatives from Fruits of Peucedanum luxurians Tamamsch. Molecules. 2018; 23(5):1222. https://doi.org/10.3390/molecules23051222

Chicago/Turabian Style

Widelski, Jarosław; Luca, Simon V.; Skiba, Adrianna; Chinou, Ioanna; Marcourt, Laurence; Wolfender, Jean-Luc; Skalicka-Wozniak, Krystyna. 2018. "Isolation and Antimicrobial Activity of Coumarin Derivatives from Fruits of Peucedanum luxurians Tamamsch" Molecules 23, no. 5: 1222. https://doi.org/10.3390/molecules23051222

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