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Molecules 2018, 23(5), 1167;

Enrichment and Purification of Total Ginkgo Flavonoid O-Glycosides from Ginkgo Biloba Extract with Macroporous Resin and Evaluation of Anti-Inflammation Activities In Vitro

College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China
Department of Pharmacy, Jiangsu Vocational College of Medicine, Yancheng 224005, Jiangsu, China
Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang, Jiangsu 222047, China
Author to whom correspondence should be addressed.
Received: 11 April 2018 / Revised: 2 May 2018 / Accepted: 7 May 2018 / Published: 13 May 2018
(This article belongs to the Special Issue Bioactives from Bioprocessing: Sources and Production)
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In the present study, the performance and separation characteristics of six macroporous resins for the enrichment and purification of total ginkgo flavonoid O-glycosides (TGFs) (quercetin (I), kaempferol (II), isorhamnetin (III)) from Ginkgo Biloba extracts (EGB) are evaluated. The adsorption and desorption properties of TGFs are studied on macroporous resins, including D101, D201, AB-8, HPD400, D301, and D311. Along with the results, AB-8 resin exhibits the best adsorption and desorption capacity for these three ginkgo flavonoid O-glycosides among the six resins. Adsorption isotherms are created on AB-8 resin and fit well to the Langmuir (R2 > 0.96) and Freundlich (R2 > 0.92, 0.3 < 1/n < 0.7) models. After the treatment with gradient elution on AB-8 resin packed chromatography column, the contents of the three main ginkgo flavonoid O-glycosides (I, II, and III) increase from 8.93%, 9.88%, and 6.11% in the extracts to 30.12%, 35.21%, and 14.14%, respectively, in the product. The recoveries of compounds I, II, and III are 88.76%, 93.78%, and 60.90%, respectively. Additionally, the anti-inflammatory effects of TGFs are evaluated in LPS-treated RAW 264.7 macrophages, and the result demonstrates that TGFs could significantly inhibit LPS-induced NO release in vitro in a dose-dependent manner compared with the control group. These findings suggest that TGFs could potentially be natural antioxidants and anti-inflammatory ingredients that could be used in pharmaceutical products and functional food additives. View Full-Text
Keywords: EGB; TGFs; macroporous resin; anti-inflammation activities EGB; TGFs; macroporous resin; anti-inflammation activities

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Zhang, L.; Wu, T.; Xiao, W.; Wang, Z.; Ding, G.; Zhao, L. Enrichment and Purification of Total Ginkgo Flavonoid O-Glycosides from Ginkgo Biloba Extract with Macroporous Resin and Evaluation of Anti-Inflammation Activities In Vitro. Molecules 2018, 23, 1167.

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