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Open AccessArticle

Molecular Sex Identification in Dioecious Hippophae rhamnoides L. via RAPD and SCAR Markers

by 1,2,3, 1,3, 1,3, 1,3, 1,3, 1,3, 1,3, 1,* and 1,2,*
1
Qinghai Key Laboratory of Qinghai-Tibet Plateau Biological Resources, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, China
2
State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining 810016, China
3
University of the Chinese Academy of Sciences, Beijing 100049, China
*
Authors to whom correspondence should be addressed.
Molecules 2018, 23(5), 1048; https://doi.org/10.3390/molecules23051048
Received: 7 April 2018 / Revised: 24 April 2018 / Accepted: 27 April 2018 / Published: 1 May 2018
The dioecious property of the sea buckthorn (Hippophae rhamnoides L.) prevents sex recognition via traditional observation at the juvenile stage, thus impeding breeding and economic cropping; A random amplified polymorphic DNA (RAPD) and a sequence characterized amplified region (SCAR) markers were used to identify the sexes. A total of 45 random decamer primers were used to screen genomic DNA pools of staminate and pistillate genotypes for genetic polymorphisms. One female sex-linked marker was identified. D15 (5′-CATCCGTGCT-3′) amplified a particular band of 885 bp, which showed polymorphism among staminate and pistillate genotype plants. The SCAR marker Hrcx-15 was obtained by sequencing the fragment. The alleles of 140 pistillate genotypes were examined but not of the 140 staminate genotypes discerned via taxonomy. Staminate and pistillate genotypes of sea buckthorn plants can be distinguished, using Hrcx-15 as a genetic marker for sex identification and for expediting cultivation for commercial applications. View Full-Text
Keywords: Hippophae rhamnoides L; dioecious; sex determination; RAPD; SCAR Hippophae rhamnoides L; dioecious; sex determination; RAPD; SCAR
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Zhou, W.; Wang, Y.; Zhang, G.; Luan, G.; Chen, S.; Meng, J.; Wang, H.; Hu, N.; Suo, Y. Molecular Sex Identification in Dioecious Hippophae rhamnoides L. via RAPD and SCAR Markers. Molecules 2018, 23, 1048.

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