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Open AccessArticle

Ectopic Overexpression of a Novel R2R3-MYB, NtMYB2 from Chinese Narcissus Represses Anthocyanin Biosynthesis in Tobacco

1
College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 35002, China
2
The New Zealand Institute for Plant & Food Research, Mt Albert Research Centre, Private Bag 92169, 1142 Auckland, New Zealand
3
School of Biological Sciences, University of Auckland, Private Bag 92019, 1142 Auckland, New Zealand
*
Author to whom correspondence should be addressed.
Molecules 2018, 23(4), 781; https://doi.org/10.3390/molecules23040781
Received: 5 March 2018 / Revised: 21 March 2018 / Accepted: 23 March 2018 / Published: 28 March 2018
(This article belongs to the Section Molecular Diversity)
R2R3 MYB transcription factors play key functions in the regulation of secondary metabolites. In the present study, a R2R3 MYB transcriptional factor NtMYB2 was identified from Chinese narcissus (Narcissus tazetta L. var. Chinensis Roem) and functionally characterized. NtMYB2 belongs to subgroup 4 of the R2R3 MYB transcription factor family that are related to repressor MYBs involved in the regulation of anthocyanin and flavonoids. Transient expression confirmed that NtMYB2 strongly reduced the red pigmentation induced by MYB- anthocyanin activators in agro-infiltrated tobacco leaves. Ectopic expression of NtMYB2 in tobacco significantly reduced the pigmentation and altered the floral phenotypes in transgenic tobacco flowers. Gene expression analysis suggested that NtMYB2 repressed the transcript levels of structural genes involved in anthocyanin biosynthesis pathway, especially the UFGT gene. NtMYB2 gene is expressed in all examined narcissus tissues; the levels of transcription in petals and corona is higher than other tissues and the transcription level at the bud stage was highest. These results show that NtMYB2 is involved in the regulation of anthocyanin biosynthesis pathway and may act as a repressor by down regulating the transcripts of key enzyme genes in Chinese narcissus. View Full-Text
Keywords: Chinese narcissus; R2R3 MYB; anthocyanin repressor; anthocyanin pathway; transcriptional regulation Chinese narcissus; R2R3 MYB; anthocyanin repressor; anthocyanin pathway; transcriptional regulation
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MDPI and ACS Style

Anwar, M.; Wang, G.; Wu, J.; Waheed, S.; Allan, A.C.; Zeng, L. Ectopic Overexpression of a Novel R2R3-MYB, NtMYB2 from Chinese Narcissus Represses Anthocyanin Biosynthesis in Tobacco. Molecules 2018, 23, 781.

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