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Molecules 2018, 23(3), 538;

Bivariate Correlation Analysis of the Chemometric Profiles of Chinese Wild Salvia miltiorrhiza Based on UPLC-Qqq-MS and Antioxidant Activities

College of Life Science, Zhejiang Sci-Tech University, Hangzhou 310018, China
Institute of Soil and Water Conservation, Chinese Academy of Sciences & Ministry of Water Resources, Yangling 712100, China
Industrial Crops Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China
Department of Research and Development, Focused Photonics Inc., Hangzhou 310018, China
Department of Physicochemical and Toxicology, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310018, China
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Received: 26 January 2018 / Revised: 21 February 2018 / Accepted: 26 February 2018 / Published: 28 February 2018
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To better understand the mechanisms underlying the pharmacological actions of Salvia miltiorrhiza, correlation between the chemical profiles and in vitro antioxidant activities in 50 batches of wild S. miltiorrhiza samples was analyzed. Our ultra-performance liquid chromatography–tandem mass spectrometry analysis detected twelve phenolic acids and five tanshinones and obtained various chemical profiles from different origins. In a principal component analysis (PCA) and cluster analysis, the tanshinones cryptotanshinone, tanshinone IIA and dihydrotanshinone I exhibited higher weights in PC1, whereas the phenolic acids danshensu, salvianolic acids A and B and lithospermic acid were highly loaded in PC2. All components could be optimized as markers of different locations and might be suitable for S. miltiorrhiza quality analyses. Additionally, the DPPH and ABTS assays used to comprehensively evaluate antioxidant activities indicated large variations, with mean DPPH and ABTS scavenging potencies of 32.24 and 23.39 μg/mL, respectively, among S. miltiorrhiza extract solutions. Notably, samples that exceeded the mean IC50 values had higher phenolic acid contents. A correlation analysis indicated a strong correlation between the antioxidant activities and phenolic acid contents. Caffeic acid, danshensu, rosmarinic acid, lithospermic acid and salvianolic acid B were major contributors to antioxidant activity. In conclusion, phenolic compounds were the predominant antioxidant components in the investigated plant species. These plants may be sources of potent natural antioxidants and beneficial chemopreventive agents. View Full-Text
Keywords: Salvia miltiorrhiza; antioxidant activities; UPLC-Qqq-MS/MS; bivariate correlation analysis Salvia miltiorrhiza; antioxidant activities; UPLC-Qqq-MS/MS; bivariate correlation analysis

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Zhang, X.; Yu, Y.; Cen, Y.; Yang, D.; Qi, Z.; Hou, Z.; Han, S.; Cai, Z.; Liu, K. Bivariate Correlation Analysis of the Chemometric Profiles of Chinese Wild Salvia miltiorrhiza Based on UPLC-Qqq-MS and Antioxidant Activities. Molecules 2018, 23, 538.

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