Trifluoromethyl Boron Dipyrromethene Derivatives as Potential Photosensitizers for Photodynamic Therapy
Abstract
:1. Introduction
2. Results and Discussion
2.1. Molecular Design and Synthesis
2.2. Photo-Physical and Photo-Chemical Properties
2.3. In Vitro Studies
2.3.1. Photocytotoxicity Studies
2.3.2. Measurements of Intracellular ROS
2.3.3. Subcellular Localization
3. Materials and Instruments
3.1. Synthesis
3.1.1. Synthesis of 1a
3.1.2. Synthesis of 1b
3.1.3. Synthesis of 2a
3.1.4. Synthesis of 2b
3.1.5. Synthesis of BDP3
3.1.6. Synthesis of BDP6
3.2. Photo-Physical and Photo-Chemical Studies
3.2.1. Absorption and Fluorescence Studies
3.2.2. Detection of Singlet Oxygen (1O2) Generation Efficiency
3.3. In Vitro Studies
3.3.1. Cell Culture and Conditions
3.3.2. Photocytotoxicity Studies
3.3.3. Measurements of Intracellular ROS
3.3.4. Subcellular Localization Studies
4. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Sample Availability: Not available. |
Compounds | λmax/nm (log ε) | λem/nm a | ΦF b | ΦΔ c |
---|---|---|---|---|
BDP3 | 638 (4.84) | 662 | 0.17 | 0.36 |
BDP6 | 640 (5.29) | 661 | 0.16 | 0.32 |
Compounds | HepG2 | HeLa |
---|---|---|
IC50 (μM) a | ||
BDP3 | 0.49 | 0.42 |
BDP6 | 0.45 | 0.45 |
Compounds | Rosup | BDP3 | BDP6 |
---|---|---|---|
Fluorescence Intensity of DCF | 4967.72 ± 154.29 | 4658.25 ± 60.60 | 4465.25 ± 164.95 |
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Liu, J.-Y.; Zhou, P.-Z.; Ma, J.-L.; Jia, X. Trifluoromethyl Boron Dipyrromethene Derivatives as Potential Photosensitizers for Photodynamic Therapy. Molecules 2018, 23, 458. https://doi.org/10.3390/molecules23020458
Liu J-Y, Zhou P-Z, Ma J-L, Jia X. Trifluoromethyl Boron Dipyrromethene Derivatives as Potential Photosensitizers for Photodynamic Therapy. Molecules. 2018; 23(2):458. https://doi.org/10.3390/molecules23020458
Chicago/Turabian StyleLiu, Jian-Yong, Peng-Zhen Zhou, Jia-Lin Ma, and Xiao Jia. 2018. "Trifluoromethyl Boron Dipyrromethene Derivatives as Potential Photosensitizers for Photodynamic Therapy" Molecules 23, no. 2: 458. https://doi.org/10.3390/molecules23020458