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Molecules 2018, 23(2), 341; https://doi.org/10.3390/molecules23020341

Modification and Assembly of a Versatile Lactonase for Bacterial Quorum Quenching

1
Institute for Bioscience and Biotechnology Research (IBBR), University of Maryland, College Park, MD 20742, USA
2
Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA
*
Author to whom correspondence should be addressed.
Received: 14 December 2017 / Revised: 23 January 2018 / Accepted: 23 January 2018 / Published: 6 February 2018
(This article belongs to the Special Issue Design and Synthesis of Quorum-Sensing Inhibitors)
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Abstract

This work sets out to provide a self-assembled biopolymer capsule activated with a multi-functional enzyme for localized delivery. This enzyme, SsoPox, which is a lactonase and phosphotriesterase, provides a means of interrupting bacterial communication pathways that have been shown to mediate pathogenicity. Here we demonstrate the capability to express, purify and attach SsoPox to the natural biopolymer chitosan, preserving its activity to “neutralize” long-chain autoinducer-1 (AI-1) communication molecules. Attachment is shown via non-specific binding and by engineering tyrosine and glutamine affinity ‘tags’ at the C-terminus for covalent linkage. Subsequent degradation of AI-1, in this case N-(3-oxododecanoyl)-l-homoserine lactone (OdDHL), serves to “quench” bacterial quorum sensing (QS), silencing intraspecies communication. By attaching enzymes to pH-responsive chitosan that, in turn, can be assembled into various forms, we demonstrate device-based flexibility for enzyme delivery. Specifically, we have assembled quorum-quenching capsules consisting of an alginate inner core and an enzyme “decorated” chitosan shell that are shown to preclude bacterial QS crosstalk, minimizing QS mediated behaviors. View Full-Text
Keywords: quorum quenching; antibiotics; organophosphate; enzyme attachment; enzyme delivery quorum quenching; antibiotics; organophosphate; enzyme attachment; enzyme delivery
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Rhoads, M.K.; Hauk, P.; Gupta, V.; Bookstaver, M.L.; Stephens, K.; Payne, G.F.; Bentley, W.E. Modification and Assembly of a Versatile Lactonase for Bacterial Quorum Quenching. Molecules 2018, 23, 341.

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