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Molecules 2018, 23(2), 156; https://doi.org/10.3390/molecules23020156

Proteomic Analysis of Differentially-Expressed Proteins in the Liver of Streptozotocin-Induced Diabetic Rats Treated with Parkia biglobosa Protein Isolate

1
Biotechnology and Structural Biology (BSB) Group, Department of Biochemistry and Microbiology, University of Zululand, KwaDlangezwa 3886, South Africa
2
Department of Biochemistry, College of Sciences, Afe Babalola University, PMB 5454, Ado-Ekiti 360001, Nigeria
*
Author to whom correspondence should be addressed.
Received: 29 October 2017 / Revised: 26 December 2017 / Accepted: 19 January 2018 / Published: 24 January 2018
(This article belongs to the Special Issue Medicinal Plants and Diabetes)
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Abstract

Protein isolate from Parkia biglobosa seeds is believed to possess excellent anti-diabetic properties. The purpose of this study was to identify differentially expressed proteins in liver of streptozotocin-induced diabetic rats treated with Parkia biglobosa seeds protein isolate (PBPi). In this study, total proteins extracted from rat liver were separated on one-dimensional SDS polyacrylamide gel (1D SDS-PAGE) and stained with Coomassie brilliant blue (CBB) to visualize protein bands. We observed that protein bands in the region of 10–15 kDa were altered by the different treatments; these bands were selected and excised for in-gel digestion and peptide extraction followed by nLC-MS, MALDI-TOF MS, and LIFT MS/MS. A database search with the Mascot algorithm positively identified four differentially expressed proteins. These proteins are known to be responsible for diverse biological functions within various organs and tissues. The present result gives insight and understanding into possible molecular mechanisms by which streptozotocin causes various alterations in proteins found in the liver of diabetic rats and the possible modulatory role of PBPi in the management of streptozotocin-induced diabetes. View Full-Text
Keywords: MALDI-TOF-MS; nLC-MS; Parkia biglobosa; proteomics; rat liver; STZ-diabetes MALDI-TOF-MS; nLC-MS; Parkia biglobosa; proteomics; rat liver; STZ-diabetes
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Ogunyinka, B.I.; Oyinloye, B.E.; Osunsanmi, F.O.; Opoku, A.R.; Kappo, A.P. Proteomic Analysis of Differentially-Expressed Proteins in the Liver of Streptozotocin-Induced Diabetic Rats Treated with Parkia biglobosa Protein Isolate. Molecules 2018, 23, 156.

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