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Molecules 2018, 23(2), 132; https://doi.org/10.3390/molecules23020132

Use of an UHPLC-MS/MS Method for Determination of Kuraridin and Characterization of Its Metabolites in Rat Plasma after Oral Administration

1
School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China
2
Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica of State Administration of TCM, China; Engineering & Technology Research Center for Chinese Materia Medical Quality of the Universities of Guangdong Province, Guangdong Pharmaceutical University, Guangzhou 510006, China
3
Department of Pharmacy, Hunan University of Medicine, Huaihua 418000, China
*
Author to whom correspondence should be addressed.
Received: 1 January 2018 / Revised: 19 January 2018 / Accepted: 19 January 2018 / Published: 24 January 2018
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Abstract

Kuraridin is an active natural prenylated flavonoid ingredient originating from the well-known traditional Chinese medicine Sophora flavescens Ait., that possesses various bioactivities, such as antitumor activity, PLCγ1 inhibitory activity, glycosidase inhibitory activity, etc. However, there is no report on the plasma metabolic profile and pharmacokinetic study of kuraridin. The current study was designed to use an ultra-performance liquid chromatography/tandem mass spectrometry (UHPLC-MS/MS) method for the quantification and characterization metabolites in rat plasma after oral administration of kuraridin. A liquid-liquid extraction method with ethyl acetate-acetonitrile (1:3) was used to extract the kuraridin from rat plasma samples. The chromatographic separation was carried out on a Hypersil GOLD UHPLC C18 column equipped with a C18 guard cartridge using a gradient elution with organic solvent-water as mobile phase. Based on comparing the retention times with reference standards or on the basis of MS2 fragmentation behaviors, a total of 19 metabolites were identified or tentatively characterized from rat plasma. Under the optimized conditions, the method showed good linearity (r2 > 0.99) over the ranges of 1–500 ng/mL for kuraridin. The inter- and intra-day precisions were less than 8.95%, and the accuracy was in the range of −6.27–6.48%. The recovery of kuraridin ranged from 90.1% to 100.4%. The developed UHPLC-MS/MS method was thus successfully applied in the qualitative of metabolites and quantitative analysis of kuraridin in rat plasma. View Full-Text
Keywords: kuraridin; UHPLC-MS/MS; rat plasma; metabolites; pharmacokinetic kuraridin; UHPLC-MS/MS; rat plasma; metabolites; pharmacokinetic
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Liu, Y.; Chen, L.; Cai, W.; Zhao, L.-L.; Mo, Z.-X. Use of an UHPLC-MS/MS Method for Determination of Kuraridin and Characterization of Its Metabolites in Rat Plasma after Oral Administration. Molecules 2018, 23, 132.

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