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Open AccessFeature PaperCommunication

Optimization of the Extraction Conditions and Biological Evaluation of Dendropanax morbifera H. Lev as an Anti-Hyperuricemic Source

1
Department of Pharmacy, College of Pharmacy, Mokpo National University, Muan, Jeonnam 58554, Korea
2
Jeonnam Institute of Natural Resources Research, Jangheung-gun, Jeonnam 57922, Korea
3
Department of Parmaceutical Engineering, Dongshin University, Naju, Jeonnam 58245, Korea
4
Department of Nursing, Dongshin University, Naju, Jeonnam 58245, Korea
*
Authors to whom correspondence should be addressed.
Academic Editors: In-Soo Yoon and Hyun-Jong Cho
Molecules 2018, 23(12), 3313; https://doi.org/10.3390/molecules23123313
Received: 1 November 2018 / Revised: 3 December 2018 / Accepted: 13 December 2018 / Published: 14 December 2018
(This article belongs to the Special Issue Method Development and Validation in Food and Pharmaceutical Analysis)
Dendropanax morbifera H. Levis a medicinal plant native to South Korea, East Asia, and South America. Among some 75 species, one species grows in Korea. In previous studies, D. morbifera extracts with anti-oxidant, anti-inflammatory, anti-complementary and anti-cancer activities were reported. The present study aims to investigate optimization of extraction and evaluation of anti-hyperuricemic effects of D. morbifera leaf and the phytochemicals contained therein. Ethanol and hexane extract were found to display the best xanthine oxidase inhibition among six types of solvent and water extract. The antioxidant effect of the ethanol extract was superior to that of the hexane extract. The DPPH radical scavenging effect of the ethanol and hexane extracts were 81.52 ± 1.57% and 2.69 ± 0.16. The reducing power of the ethanol and hexane extracts were 9.71 ± 0.15 and 0.89 ± 0.01 mg/g equivalent of gallic acid. Total phenols of the ethanol and hexane extracts were 6.53 ± 0.16 and 0.63 ± 0.001 mg/g equivalent of gallic acid. In addition, we compared the two marker compounds from D. morbifera, chlorogenic acid and rutin, which were determined in the ethanol extract at 0.80 ± 0.03% and 0.52 ± 0.01%, respectively. We found that the ethanol extracts showed better xanthine oxidase inhibition than hexane extracts. Especially, ethanol extracts showed higher antioxidant activity than hexane extracts. Based on these results, we selected the ethanol extract as an effective xanthine oxidase inhibitor and confirmed whether ethanol extracts showed xanthine oxidase inhibition in animal experiments. The in vivo mouse study demonstrated that ethanol extract of D. morbifera leaf at the dose of 300 mg/kg could inhibit blood/hepatic xanthine oxidase activity and this result shows that the xanthine oxidase inhibitory activity in vitro is reproduced in vivo. The present study showed that ethanol extract was optimal xanthine oxidase inhibitor which can be applied to prevent diseases related to hyperuricemia. View Full-Text
Keywords: Dendropanax morbifera leaf; xanthine oxidase; hyperuricemia; HPLC Dendropanax morbifera leaf; xanthine oxidase; hyperuricemia; HPLC
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Cho, S.-S.; Song, S.-H.; Choi, C.-Y.; Park, K.M.; Shim, J.-H.; Park, D.-H. Optimization of the Extraction Conditions and Biological Evaluation of Dendropanax morbifera H. Lev as an Anti-Hyperuricemic Source. Molecules 2018, 23, 3313.

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