Extraction, Characterization and Antioxidant Activity in vitro of Proteins from Semen Allii Fistulosi
Abstract
:1. Introduction
2. Results and Discussion
2.1. PSAF Extraction Process Parameters
2.2. Optimization for PSAF Extraction
2.3. Characterization of PSAF
2.4. Antioxidant Activities in vitro
3. Materials and Methods
3.1. Materials and Equipment
3.2. Sample Pretreatment
3.3. Protein Content Determination
3.4. Extraction Optimization
3.5. Characterization of PSAF Isoelectric Point and Amino Acid Composition
3.6. SDS-PAGE and FT-IR Spectra Analysis
3.7. DPPH Radical Scavenging Assay
3.8. Hydroxyl and Superoxide Radical Scavenging Activity
3.9. Fe2+ Capacity Activity and Reducing Power Determination
3.10. Statistical Analysis
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Sample Availability: Samples of the compounds are not available from the authors. |
No. | pH (A) | Extraction Time (B) | Liquid-to-Solid Ratio (C) | Temperature (D) | Yield of PSAF (%) |
---|---|---|---|---|---|
1 | 1 | 1 | 1 | 1 | 4.41 |
2 | 1 | 2 | 2 | 2 | 4.86 |
3 | 1 | 3 | 3 | 3 | 6.29 |
4 | 2 | 1 | 3 | 3 | 4.67 |
5 | 2 | 2 | 1 | 1 | 4.94 |
6 | 2 | 3 | 2 | 2 | 4.63 |
7 | 3 | 1 | 2 | 2 | 6.11 |
8 | 3 | 2 | 3 | 3 | 4.75 |
9 | 3 | 3 | 1 | 1 | 4.52 |
K1 | 5.19 | 5.06 | 4.60 | 4.62 | |
K2 | 4.74 | 4.85 | 4.68 | 5.20 | |
K3 | 5.13 | 5.15 | 5.78 | 5.24 | |
R | 0.44 | 0.30 | 1.18 | 0.62 |
Amino Acid | PSAF | FAO/WHO | Amino Acid | PSAF | FAO/WHO |
---|---|---|---|---|---|
Aspartic acid | 6.33 | - | Valine | 4.49 | 4.96 |
Serine | 4.47 | - | Cysteine & methionine | 5.44 | 3.52 |
Glutamic acid | 15.27 | - | Isoleucine | 2.01 | 4.0 |
Glycine | 4.2 | - | Leucine | 4.43 | 7.04 |
Alanine | 4.88 | - | Phenylalanine & tyrosine | 6.74 | 6.08 |
Histidine | 1.69 | - | Lysine | 6.22 | 5.44 |
Arginine | 8.78 | - | FLAA | Ile | - |
Proline | 2.26 | - | SLAA | Leu | - |
Threonine | 2.81 | 4.0 |
Factor Level | pH | Extraction Time (min) | Liquid-to-Solid Ratio | Temperature (°C) |
---|---|---|---|---|
1 | 8.0 | 90 | 25:1 | 40 |
2 | 8.5 | 120 | 30:1 | 50 |
3 | 9.0 | 150 | 35:1 | 60 |
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Zuo, M.; Liu, X.-x.; Liu, D.; Zhao, H.-y.; Xuan, L.-l.; Jiang, W.-x.; Li, W.-z. Extraction, Characterization and Antioxidant Activity in vitro of Proteins from Semen Allii Fistulosi. Molecules 2018, 23, 3235. https://doi.org/10.3390/molecules23123235
Zuo M, Liu X-x, Liu D, Zhao H-y, Xuan L-l, Jiang W-x, Li W-z. Extraction, Characterization and Antioxidant Activity in vitro of Proteins from Semen Allii Fistulosi. Molecules. 2018; 23(12):3235. https://doi.org/10.3390/molecules23123235
Chicago/Turabian StyleZuo, Min, Xiao-xiao Liu, Di Liu, Hang-yun Zhao, Lu-lu Xuan, Wen-xian Jiang, and Wan-zhong Li. 2018. "Extraction, Characterization and Antioxidant Activity in vitro of Proteins from Semen Allii Fistulosi" Molecules 23, no. 12: 3235. https://doi.org/10.3390/molecules23123235