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Open AccessArticle

A Novel Method for the Quantification of White Wine Mannoproteins by a Competitive Indirect Enzyme-Linked Lectin Sorbent Assay (CI-ELLSA)

1
Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padova, Viale dell’Università 16, 35020 Padova, Italy
2
Centre for Research in Viticulture and Enology (CIRVE), Viale XXVIII Aprile 14, 31015 Conegliano, Italy
*
Author to whom correspondence should be addressed.
Molecules 2018, 23(12), 3070; https://doi.org/10.3390/molecules23123070
Received: 4 October 2018 / Revised: 12 November 2018 / Accepted: 20 November 2018 / Published: 23 November 2018
(This article belongs to the Collection Wine Chemistry)
Mannoproteins (MPs) are cell wall proteoglycans released in wine by yeast during fermentation and ageing on lees, a procedure used for the production of several wines to enrich them in these components with consequences from both a technological and sensory point of view. Given the significance that wine MPs have for wine quality, winemakers would welcome a simple and accurate method for their quantification, as this would allow them to have a better control of this aspect at different winemaking stages. This study develops and validates a novel, simple and accurate method for MPs quantification in white wines based on a competitive indirect enzyme-linked lectin sorbent assay (CI-ELLSA), using the highly mannosylated yeast invertase as the standard. The method utilizes the lectin concanavalin A (ConA) as the immobilized ligand for MPs, and peroxidase, an enzyme rich in mannose, as the competitor for ConA. After addition of the peroxidase substrate, the intensity of the signal produced by the activity of this enzyme (absorbance at 450 nm) is inversely proportional to the amount of mannosylated proteins in the sample. Results have been validated on several wine styles including still, sparkling and sweet wines. View Full-Text
Keywords: wine; mannoproteins; competitive indirect enzyme-linked lectin sorbent assay (CI-ELLSA); method; quantification; yeast invertase wine; mannoproteins; competitive indirect enzyme-linked lectin sorbent assay (CI-ELLSA); method; quantification; yeast invertase
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MDPI and ACS Style

Marangon, M.; Vegro, M.; Vincenzi, S.; Lomolino, G.; De Iseppi, A.; Curioni, A. A Novel Method for the Quantification of White Wine Mannoproteins by a Competitive Indirect Enzyme-Linked Lectin Sorbent Assay (CI-ELLSA). Molecules 2018, 23, 3070.

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