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Molecules 2018, 23(11), 3007;

DNA Replication: From Radioisotopes to Click Chemistry

Faculty of Medicine and Dentistry, Institute of Molecular and Translational Medicine, Palacký University in Olomouc, Hněvotínská 5, 779 00 Olomouc, Czech Republic
Authors to whom correspondence should be addressed.
Academic Editor: Arnaud Gautier
Received: 29 October 2018 / Revised: 14 November 2018 / Accepted: 15 November 2018 / Published: 17 November 2018
(This article belongs to the Collection Advances in Click Chemistry)
Full-Text   |   PDF [3125 KB, uploaded 17 November 2018]   |  


The replication of nuclear and mitochondrial DNA are basic processes assuring the doubling of the genetic information of eukaryotic cells. In research of the basic principles of DNA replication, and also in the studies focused on the cell cycle, an important role is played by artificially-prepared nucleoside and nucleotide analogues that serve as markers of newly synthesized DNA. These analogues are incorporated into the DNA during DNA replication, and are subsequently visualized. Several methods are used for their detection, including the highly popular click chemistry. This review aims to provide the readers with basic information about the various possibilities of the detection of replication activity using nucleoside and nucleotide analogues, and to show the strengths and weaknesses of those different detection systems, including click chemistry for microscopic studies. View Full-Text
Keywords: click chemistry; nucleoside and nucleotide analogues; indirect immunocytochemistry; isotopes click chemistry; nucleoside and nucleotide analogues; indirect immunocytochemistry; isotopes

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Ligasová, A.; Koberna, K. DNA Replication: From Radioisotopes to Click Chemistry. Molecules 2018, 23, 3007.

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