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Open AccessArticle

Identification of Anti-Melanogenesis Constituents from Morus alba L. Leaves

1
College of Pharmacy, Chungnam National University, Daejeon 34134, Korea
2
College of Pharmacy and Research Institute of Drug Development, Chonnam National University, Gwangju 61186, Korea
3
Division of Applied Bioengineering, College of Engineering, Dong-Eui University, Busan 47340, Korea
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Isabel C. F. R. Ferreira
Molecules 2018, 23(10), 2559; https://doi.org/10.3390/molecules23102559
Received: 23 August 2018 / Revised: 29 September 2018 / Accepted: 30 September 2018 / Published: 8 October 2018
(This article belongs to the Collection Bioactive Compounds)
The individual parts of Morus alba L. including root bark, branches, leaves, and fruits are used as a cosmetic ingredient in many Asian countries. This study identified several anti-melanogenesis constituents in a 70% ethanol extract of M. alba leaves. The ethyl acetate fraction of the initial ethanol extract decreased the activity of tyrosinase, a key enzyme in the synthetic pathway of melanin. Twelve compounds were isolated from this fraction and their structures were identified based on spectroscopic spectra. Then, the authors investigated the anti-melanogenesis effects of the isolated compounds in B16-F10 mouse melanoma cells. Compounds 3 and 8 significantly inhibited not only melanin production but also intracellular tyrosinase activity in alpha-melanocyte-stimulating-hormone (α-MSH)-induced B16-F10 cells in a dose-dependent manner. These same compounds also inhibited melanogenesis-related protein expression such as microphthalmia-associated transcription factor (MITF), tyrosinase, and tyrosinase-related protein-1 (TRP-1). Compound 3 modulated the cAMP-responsive element-binding protein (CREB) and p38 signaling pathways in α-MSH-activated B16-F10 melanoma cells, which resulted in the anti-melanogenesis effects. These results suggest that compound 3, isolated from M. alba leaves, could be used to inhibit melanin production via the regulation of melanogenesis-related protein expression. View Full-Text
Keywords: Morus alba leaf; anti-melanogenesis; MITF; tyrosinase; TRP-1; TRP-2 Morus alba leaf; anti-melanogenesis; MITF; tyrosinase; TRP-1; TRP-2
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MDPI and ACS Style

Li, H.X.; Park, J.U.; Su, X.D.; Kim, K.T.; Kang, J.S.; Kim, Y.R.; Kim, Y.H.; Yang, S.Y. Identification of Anti-Melanogenesis Constituents from Morus alba L. Leaves. Molecules 2018, 23, 2559. https://doi.org/10.3390/molecules23102559

AMA Style

Li HX, Park JU, Su XD, Kim KT, Kang JS, Kim YR, Kim YH, Yang SY. Identification of Anti-Melanogenesis Constituents from Morus alba L. Leaves. Molecules. 2018; 23(10):2559. https://doi.org/10.3390/molecules23102559

Chicago/Turabian Style

Li, Hong X.; Park, Jung U.; Su, Xiang D.; Kim, Kyung T.; Kang, Jong S.; Kim, Young R.; Kim, Young H.; Yang, Seo Y. 2018. "Identification of Anti-Melanogenesis Constituents from Morus alba L. Leaves" Molecules 23, no. 10: 2559. https://doi.org/10.3390/molecules23102559

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