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Article

UPLC-MS/MS Method for the Determination of 14 Compounds in Rat Plasma and Its Application in a Pharmacokinetic Study of Orally Administered Xiaoyao Powder

1
Key Laboratory of Photochemistry Biomaterials and Energy Storage Materials of Heilongjiang Province, College of Chemistry & Chemical Engineering, Harbin Normal University, No.1 South Road, Limin Development Zone, Harbin 150025, China
2
College of Jiamusi, Heilongjiang University of Chinese Medicine, Jiamusi, Heilongjiang 154007, China
3
Crop Academy of Heilongjiang University, Harbin 150080, China
*
Authors to whom correspondence should be addressed.
Molecules 2018, 23(10), 2514; https://doi.org/10.3390/molecules23102514
Received: 9 September 2018 / Revised: 25 September 2018 / Accepted: 26 September 2018 / Published: 30 September 2018
(This article belongs to the Special Issue Natural Product Pharmacology and Medicinal Chemistry)
Xiaoyao Powder (XYP), a common Chinese medicine, comprises eight traditional Chinese herbs and has been widely used clinically to treat liver damage and mental disorders. An ultra-performance liquid chromatography–tandem mass spectrometry method was developed to investigate the pharmacokinetics of 14 compounds (albiflorin, paeoniflorin, ferulic acid, senkyunolide I, quercetin, isoliquiritigenin, atractylenolide III, ligustilide, atractylenolide II, liquiritin, liquiritigenin, saikosaponin c, glycyrrhizic acid, and saikosaponin a) in XYP. Naringenin was used as the internal standard. The compounds were separated using an ACQUITY UPLCTM BEH C18 column (1.7 μm, 50 × 2.1 mm) with a mobile phase consisting of acetonitrile and 0.1% formic acid in water at a flow rate of 0.3 mL/min. Detection was performed on a triple-quadrupole tandem mass spectrometer using multiple reaction monitoring and an electrospray ionization source in both positive and negative ionization modes. All calibration curves exhibited good linearity (r2 > 0.9974) over the measured ranges. The intra- and inter-day precisions were within 12%, and the accuracy ranged from 89.93% to 106.64%. Extraction recovery and matrix effect results were satisfactory. The method was successfully applied in a pharmacokinetic study of the 14 compounds in rat plasma after the oral administration of XYP. View Full-Text
Keywords: Xiaoyao Powder; pharmacokinetics; UPLC-MS/MS; rat plasma; oral administration Xiaoyao Powder; pharmacokinetics; UPLC-MS/MS; rat plasma; oral administration
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MDPI and ACS Style

Xu, M.; Xu, Z.; Xu, Q.; Zhang, H.; Liu, M.; Geng, F.; Zhang, N. UPLC-MS/MS Method for the Determination of 14 Compounds in Rat Plasma and Its Application in a Pharmacokinetic Study of Orally Administered Xiaoyao Powder. Molecules 2018, 23, 2514. https://doi.org/10.3390/molecules23102514

AMA Style

Xu M, Xu Z, Xu Q, Zhang H, Liu M, Geng F, Zhang N. UPLC-MS/MS Method for the Determination of 14 Compounds in Rat Plasma and Its Application in a Pharmacokinetic Study of Orally Administered Xiaoyao Powder. Molecules. 2018; 23(10):2514. https://doi.org/10.3390/molecules23102514

Chicago/Turabian Style

Xu, Mingyue, Zhanling Xu, Qingxuan Xu, Hongyue Zhang, Mingyang Liu, Fang Geng, and Ning Zhang. 2018. "UPLC-MS/MS Method for the Determination of 14 Compounds in Rat Plasma and Its Application in a Pharmacokinetic Study of Orally Administered Xiaoyao Powder" Molecules 23, no. 10: 2514. https://doi.org/10.3390/molecules23102514

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