Molecules 2018, 23(1), 197; https://doi.org/10.3390/molecules23010197
β-Escin Effectively Modulates HUVECs Proliferation and Tube Formation
Lenka Varinská 1,2,†, Lenka Fáber 1,†, Martin Kello 1, Eva Petrovová 3, Ľudmila Balážová 4, Peter Solár 5
, Matúš Čoma 1, Peter Urdzík 6, Ján Mojžiš 1, Emil Švajdlenka 7,8, Pavel Mučaji 9 and Peter Gál 1,2,9,*
, Matúš Čoma 1, Peter Urdzík 6, Ján Mojžiš 1, Emil Švajdlenka 7,8, Pavel Mučaji 9 and Peter Gál 1,2,9,*
1
Department of Pharmacology, Faculty of Medicine, Pavol Jozef Šafárik University, 040 11 Košice, Slovakia
2
Department of Biomedical Research, East-Slovak Institute of Cardiovascular Diseases, Inc., 040 11 Košice, Slovakia
3
Department of Anatomy, Histology and Physiology, University of Veterinary Medicine and Pharmacy, 040 11 Košice, Slovakia
4
Department of Pharmacognosy and Botany, University of Veterinary Medicine and Pharmacy, 041 81 Košice, Slovakia
5
Department of Medical Biology, Faculty of Medicine, Pavol Jozef Šafárik University, 040 11 Košice, Slovakia
6
Department of Gynaecology and Obstetrics, Faculty of Medicine, Pavol Jozef Šafárik University, 040 11 Košice, Slovakia
7
Department of Chemical Theory of Drugs, Faculty of Pharmacy, Comenius University, 831 04 Bratislava, Slovakia
8
Eurofins SK, Testing Laboratory Bratislava, 811 07 Bratislava, Slovakia
9
Department of Pharmacognosy and Botany, Faculty of Pharmacy, Comenius University, 831 04 Bratislava, Slovakia
†
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 21 December 2017 / Revised: 11 January 2018 / Accepted: 12 January 2018 / Published: 17 January 2018
(This article belongs to the Special Issue Selected Papers from the 46th EuroCongress on Drug Synthesis and Analysis (ECDSA-2017))
Abstract
In the present study we evaluated the anti-angiogenic activities of β-escin (the major active compound of Aesculus hippocastanum L. seeds). Human umbilical-vein endothelial cells (HUVECs) were used as an in vitro model for studying the molecular mechanism underlying the anti-angiogenic effect of β-escin. We investigated the in vitro effects on proliferation, migration, and tube formation of HUVECs and in vivo anti-angiogenic activity was evaluated in a chick chorioallantoic membrane (CAM) angiogenesis assay. Moreover, the effect on gene expressions was determined by the RT2 ProfilerTM human angiogenesis PCR Array. It was found that β-escin exerts inhibitory effect on the basic fibroblast growth factor (bFGF)-induced proliferation, migration and tube formation, as well as CAM angiogenesis in vivo. The inhibition of critical steps of angiogenic process observed with β-escin could be partially explained by suppression of Akt activation in response to bFGF. Moreover, the anti-angiogenic effects of β-escin could also be mediated via inhibition of EFNB2 and FGF-1 gene expressions in endothelial cells. In conclusion, β-escin affects endothelial cells as a negative mediator of angiogenesis in vitro and in vivo and may therefore be considered as a promising candidate for further research elucidating its underlying mechanism of action. View Full-TextKeywords:
β-escin; HUVECs; angiogenesis; bFGF; CAM
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Varinská, L.; Fáber, L.; Kello, M.; Petrovová, E.; Balážová, Ľ.; Solár, P.; Čoma, M.; Urdzík, P.; Mojžiš, J.; Švajdlenka, E.; Mučaji, P.; Gál, P. β-Escin Effectively Modulates HUVECs Proliferation and Tube Formation. Molecules 2018, 23, 197.
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