Characterization of New Bioactive Enzyme Inhibitors from Endophytic Bacillus amyloliquefaciens RWL-1
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bacterial Growth Conditions
2.2. Extraction for Secondary Metabolite Identification
2.3. Secondary Metabolite Isolation
2.4. Reverse-Phase HPLC Analysis
2.5. Structure Elucidation of Compound 1
2.6. In Vitro Biological Activities of RWL-1 Crude Extract
2.6.1. Urease Inhibition
2.6.2. α-Glucosidase Inhibition
2.6.3. Anticancer Assay (MTT)
2.6.4. AChE Inhibition
2.7. Statistical Analysis
3. Results and Discussion
3.1. Biological Potential of RWL-1 Crude Extract
3.2. Structural Elucidation of Compound 1
3.3. Biological Evaluation of Compound 1
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Sample Availability: Samples of the compound are available from the authors. |
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Shahzad, R.; Latif Khan, A.; Ali, L.; Bilal, S.; Imran, M.; Choi, K.-S.; Al-Harrasi, A.; Lee, I.-J. Characterization of New Bioactive Enzyme Inhibitors from Endophytic Bacillus amyloliquefaciens RWL-1. Molecules 2018, 23, 114. https://doi.org/10.3390/molecules23010114
Shahzad R, Latif Khan A, Ali L, Bilal S, Imran M, Choi K-S, Al-Harrasi A, Lee I-J. Characterization of New Bioactive Enzyme Inhibitors from Endophytic Bacillus amyloliquefaciens RWL-1. Molecules. 2018; 23(1):114. https://doi.org/10.3390/molecules23010114
Chicago/Turabian StyleShahzad, Raheem, Abdul Latif Khan, Liaqat Ali, Saqib Bilal, Muhammad Imran, Kyung-Sook Choi, Ahmed Al-Harrasi, and In-Jung Lee. 2018. "Characterization of New Bioactive Enzyme Inhibitors from Endophytic Bacillus amyloliquefaciens RWL-1" Molecules 23, no. 1: 114. https://doi.org/10.3390/molecules23010114