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Molecules 2017, 22(6), 962;

Subcritical Water Chromatography with Electrochemical Detection

Department of Chemistry, East Carolina University, Science & Technology Building 584, Greenville, NC 27858, USA
Author to whom correspondence should be addressed.
Received: 9 May 2017 / Revised: 3 June 2017 / Accepted: 7 June 2017 / Published: 9 June 2017
(This article belongs to the Special Issue Sub- and Supercritical Fluids and Green Chemistry)
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Reverse phase liquid chromatography (RPLC) is a commonly used separation and analysis technique. RPLC typically employs mixtures of organic solvents and water or aqueous buffers as the mobile phase. With RPLC being used on a global scale, enormous quantities of organic solvents are consumed every day. In addition to the purchasing cost of the hazardous solvents, the issue of waste disposal is another concern. At ambient temperature, water is too polar to dissolve many organic substances. Therefore, although water is nontoxic it cannot be used to replace the mobile phase in RPLC since organic analytes will not be eluted. Subcritical water chromatography may be an alternative. The characteristics of water, such as polarity, surface tension, and viscosity, can be altered by manipulating water’s temperature, thus making it behave like an organic solvent. The aim of this study was to evaluate the feasibility of separation using water mobile phase and detection by an electrochemical (EC) detector. The classes of analytes studied were neurotransmitters/metabolites, nucleic acids/heterocyclic bases, and capsaicinoids. Both isothermal and temperature-programmed separations were carried out. The separation temperature ranged from 25 to 100 °C. For separations of all three classes of solutes, the retention time was decreased with increasing temperature, thus shortening the analysis time. The peaks also became narrower as temperature increased. The limit of detection of neurotransmitters/metabolites ranges from 0.112 to 0.224 ppm. View Full-Text
Keywords: subcritical water chromatography; hot water chromatography; electrochemical detection; neurotransmitters; nucleic acids; heterocyclic bases; capsaicinoids subcritical water chromatography; hot water chromatography; electrochemical detection; neurotransmitters; nucleic acids; heterocyclic bases; capsaicinoids

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Anderson, H.; Yang, Y. Subcritical Water Chromatography with Electrochemical Detection. Molecules 2017, 22, 962.

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