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Isoprenoids Production from Lipid-Extracted Microalgal Biomass Residues Using Engineered E. coli

Key Lab of Applied Mycology, College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, China
Author to whom correspondence should be addressed.
Academic Editors: Robert M. Coates and Derek J. McPhee
Molecules 2017, 22(6), 960;
Received: 20 April 2017 / Revised: 1 June 2017 / Accepted: 7 June 2017 / Published: 9 June 2017
(This article belongs to the Special Issue Isoprenoid Biosynthesis)
PDF [2364 KB, uploaded 9 June 2017]


Microalgae are recognized as a third generation feedstock for biofuel production due to their rapid growth rates and lignin-free characteristics. In this study, a lipid extracted microalgal biomass residues was used as the raw material to produce isoprene, α-pinene and β-pinene with an engineered E. coli strain. We adopted an optimal sulfuric acid hydrolysis method (1:7 ratio of solid to acid solution, 32% (w/v) concentration of sulfuric acid solution at 90 °C for 90 min) to efficiently convert holocellulose into glucose efficiently (6.37 g/L). Futhermore, we explored a novel detoxification strategy (phosphoric acid/calcium hydroxide) to remove inhibitors and notably acetic acid, furfural and 5-hydroxymethylfurfural (5-HMF) were reduced by 5.32%, different number given later 99.19% and 98.22%, respectively. Finally, the fermentation concentrations of isoprene (223.23 mg/L), α-pinene (382.21 μg/L) and β-pinene (17.4 mg/L) were achieved using the detoxified hydrolysate as the carbon source, equivalent to approximately 86.02%, 90.16% and 88.32% of those produced by the engineered E. coli strain fermented on pure glucose, respectively. View Full-Text
Keywords: lipid extracted microalgae; isoprenoids; detoxification lipid extracted microalgae; isoprenoids; detoxification

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Wang, S.; Yang, J. Isoprenoids Production from Lipid-Extracted Microalgal Biomass Residues Using Engineered E. coli. Molecules 2017, 22, 960.

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