Next Article in Journal
The Architecture of Thiol Antioxidant Systems among Invertebrate Parasites
Next Article in Special Issue
Immobilization of Lipase from Penicillium sp. Section Gracilenta (CBMAI 1583) on Different Hydrophobic Supports: Modulation of Functional Properties
Previous Article in Journal
Synthesis of Some New 1,3,4-Thiadiazole, Thiazole and Pyridine Derivatives Containing 1,2,3-Triazole Moiety
Previous Article in Special Issue
Immobilized Lipases on Functionalized Silica Particles as Potential Biocatalysts for the Synthesis of Fructose Oleate in an Organic Solvent/Water System
 
Search for Articles:
Title / Keyword
Author / Affiliation
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
Journals
Molecules
Volume 22
Issue 2
10.3390/molecules22020269
molecules-logo
Submit to this Journal Review for this Journal Edit a Special Issue
► Article Menu

Article Menu

share announcement question_answer thumb_up
...
textsms
...
Article

Novel Magnetic Cross-Linked Cellulase Aggregates with a Potential Application in Lignocellulosic Biomass Bioconversion

by
Junqi Jia
1,
Weiwei Zhang
2,
Zengjie Yang
1,
Xianling Yang
1,
Na Wang
1,* and
Xiaoqi Yu
1,*
1
Key Laboratory of Green Chemistry Technology, Ministry of Education, College of Chemistry, Sichuan University, Chengdu 610064, China
2
School of Chemistry and Chemical Engineering, Ningxia University, Yinchuan 750021, China
*
Authors to whom correspondence should be addressed.
Molecules 2017, 22(2), 269; https://doi.org/10.3390/molecules22020269
Received: 12 December 2016 / Accepted: 2 February 2017 / Published: 10 February 2017
(This article belongs to the Special Issue Enzyme Immobilization 2016)
View Full-Text Download PDF
Browse Figures

Abstract

The utilization of renewable biomass resources to produce high-value chemicals by enzymatic processes is beneficial for alternative energy production, due to the accelerating depletion of fossil fuels. As immobilization techniques can improve enzyme stability and reusability, a novel magnetic cross-linked cellulase aggregate has been developed and applied for biomass bioconversion. The crosslinked aggregates could purify and immobilize enzymes in a single operation, and could then be combined with magnetic nanoparticles (MNPs), which provides easy separation of the materials. The immobilized cellulase showed a better activity at a wider temperature range and pH values than that of the free cellulase. After six cycles of consecutive reuse, the immobilized cellulase performed successful magnetic separation and retained 74% of its initial activity when carboxylmethyl cellulose (CMC) was used as the model substrate. Furthermore, the structure and morphology of the immobilized cellulase were studied by Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). Moreover, the immobilized cellulase was shown to hydrolyze bamboo biomass with a yield of 21%, and was re-used in biomass conversion up to four cycles with 38% activity retention, which indicated that the immobilized enzyme has good potential for biomass applications. View Full-Text
Keywords: cellulase; magnetic nanoparticles; cross-linked enzyme aggregates; hydrolysis; biomass cellulase; magnetic nanoparticles; cross-linked enzyme aggregates; hydrolysis; biomass
►▼ Show Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
SciFeed

Share and Cite

MDPI and ACS Style

Jia, J.; Zhang, W.; Yang, Z.; Yang, X.; Wang, N.; Yu, X. Novel Magnetic Cross-Linked Cellulase Aggregates with a Potential Application in Lignocellulosic Biomass Bioconversion. Molecules 2017, 22, 269. https://doi.org/10.3390/molecules22020269

AMA Style

Jia J, Zhang W, Yang Z, Yang X, Wang N, Yu X. Novel Magnetic Cross-Linked Cellulase Aggregates with a Potential Application in Lignocellulosic Biomass Bioconversion. Molecules. 2017; 22(2):269. https://doi.org/10.3390/molecules22020269

Chicago/Turabian Style

Jia, Junqi, Weiwei Zhang, Zengjie Yang, Xianling Yang, Na Wang, and Xiaoqi Yu. 2017. "Novel Magnetic Cross-Linked Cellulase Aggregates with a Potential Application in Lignocellulosic Biomass Bioconversion" Molecules 22, no. 2: 269. https://doi.org/10.3390/molecules22020269

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Metrics

Article Access Map by Country/Region

1
Back to TopTop