Four New Compounds Obtained from Cultured Cells of Artemisia annua
1
Biotechnological Institute of Chinese Materia Medical, College of Pharmacy, Jinan University, Guangzhou 510632, China
2
Department of Natural Products Chemistry, College of Pharmacy, Jinan University, Guangzhou 510632, China
*
Authors to whom correspondence should be addressed.
Molecules 2017, 22(12), 2264; https://doi.org/10.3390/molecules22122264
Received: 11 November 2017
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Revised: 6 December 2017
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Accepted: 15 December 2017
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Published: 18 December 2017
(This article belongs to the Section Bioorganic Chemistry)
Four new compounds obtained from cultured cells of Artemisia annua were reported. Products were detected by HPLC-ELSD/GC-MS and isolated by chromatographic methods. The structures of four new compounds, namely 6-hydroxy arteannuin I (1), 1-hydroxy arteannuin I (2), 2-hydroxy arteannuin J (3), and 14-hydroxy arteannuin J (4), were elucidated using their physico-chemical properties by NMR and MS data analyses. The results from the spontaneous oxidative experiment indicated that the biosynthesis of the new compounds was enzyme-catalyzed. Interestingly, the enzymes in the cultured cells of A. annua showed the abilities of substrate-selective and region-selective hydroxylation of the sesquiterpene lactone. Furthermore, the artemisinin contents were increased by 50% and 80% compared to the control group after the addition of arteannuin I/J to the suspension-cultured cells of A. annua under light and dark culture conditions, respectively.
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Keywords:
Artemisia annua cultured cells; arteannuin I; arteannuin J; biosynthesis; selective hydroxylation
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MDPI and ACS Style
Zhu, J.; Xiao, P.; Qian, M.; Chen, C.; Liang, C.; Zi, J.; Yu, R. Four New Compounds Obtained from Cultured Cells of Artemisia annua. Molecules 2017, 22, 2264. https://doi.org/10.3390/molecules22122264
AMA Style
Zhu J, Xiao P, Qian M, Chen C, Liang C, Zi J, Yu R. Four New Compounds Obtained from Cultured Cells of Artemisia annua. Molecules. 2017; 22(12):2264. https://doi.org/10.3390/molecules22122264
Chicago/Turabian StyleZhu, Jianhua, Peijie Xiao, Minghua Qian, Chang Chen, Chuxin Liang, Jiachen Zi, and Rongmin Yu. 2017. "Four New Compounds Obtained from Cultured Cells of Artemisia annua" Molecules 22, no. 12: 2264. https://doi.org/10.3390/molecules22122264
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