Next Article in Journal
Optimizing the Maximum Recovery of Dihydromyricetin from Chinese Vine Tea, Ampelopsis grossedentata, Using Response Surface Methodology
Previous Article in Journal
Synthesis and Biocompatibility Studies of New Iminodiacetic Acid Derivatives
Article Menu
Issue 12 (December) cover image

Export Article

Open AccessArticle
Molecules 2017, 22(12), 2264; https://doi.org/10.3390/molecules22122264

Four New Compounds Obtained from Cultured Cells of Artemisia annua

1
Biotechnological Institute of Chinese Materia Medical, College of Pharmacy, Jinan University, Guangzhou 510632, China
2
Department of Natural Products Chemistry, College of Pharmacy, Jinan University, Guangzhou 510632, China
*
Authors to whom correspondence should be addressed.
Received: 11 November 2017 / Revised: 6 December 2017 / Accepted: 15 December 2017 / Published: 18 December 2017
(This article belongs to the Section Bioorganic Chemistry)
Full-Text   |   PDF [5699 KB, uploaded 19 December 2017]   |  

Abstract

Four new compounds obtained from cultured cells of Artemisia annua were reported. Products were detected by HPLC-ELSD/GC-MS and isolated by chromatographic methods. The structures of four new compounds, namely 6-hydroxy arteannuin I (1), 1-hydroxy arteannuin I (2), 2-hydroxy arteannuin J (3), and 14-hydroxy arteannuin J (4), were elucidated using their physico-chemical properties by NMR and MS data analyses. The results from the spontaneous oxidative experiment indicated that the biosynthesis of the new compounds was enzyme-catalyzed. Interestingly, the enzymes in the cultured cells of A. annua showed the abilities of substrate-selective and region-selective hydroxylation of the sesquiterpene lactone. Furthermore, the artemisinin contents were increased by 50% and 80% compared to the control group after the addition of arteannuin I/J to the suspension-cultured cells of A. annua under light and dark culture conditions, respectively. View Full-Text
Keywords: Artemisia annua cultured cells; arteannuin I; arteannuin J; biosynthesis; selective hydroxylation Artemisia annua cultured cells; arteannuin I; arteannuin J; biosynthesis; selective hydroxylation
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Zhu, J.; Xiao, P.; Qian, M.; Chen, C.; Liang, C.; Zi, J.; Yu, R. Four New Compounds Obtained from Cultured Cells of Artemisia annua. Molecules 2017, 22, 2264.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top