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Article

Analysis of Non-Volatile Chemical Constituents of Menthae Haplocalycis Herba by Ultra-High Performance Liquid Chromatography-High Resolution Mass Spectrometry

by
Lu-Lu Xu
1,
Jing-Jing Xu
1,
Kun-Rui Zhong
1,
Zhan-Peng Shang
1,
Fei Wang
1,
Ru-Feng Wang
1,
Le Zhang
1,
Jia-Yu Zhang
2,* and
Bin Liu
1,*
1
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100029, China
2
Beijing Research Institution of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China
*
Authors to whom correspondence should be addressed.
Molecules 2017, 22(10), 1756; https://doi.org/10.3390/molecules22101756
Submission received: 10 September 2017 / Revised: 15 October 2017 / Accepted: 16 October 2017 / Published: 19 October 2017
(This article belongs to the Special Issue New Methodologies in Natural Product Analytics and Structure Elucidation)
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Abstract

Menthae Haplocalycis herba, one kind of Chinese edible herbs, has been widely utilized for the clinical use in China for thousands of years. Over the last decades, studies on chemical constituents of Menthae Haplocalycis herba have been widely performed. However, less attention has been paid to non-volatile components which are also responsible for its medical efficacy than the volatile constituents. Therefore, a rapid and sensitive method was developed for the comprehensive identification of the non-volatile constituents in Menthae Haplocalycis herba using ultra-high performance liquid chromatography coupled with linear ion trap-Orbitrap mass spectrometry (UHPLC-LTQ-Orbitrap). Separation was performed with Acquity UPLC® BEH C18 column (2.1 mm × 100 mm, 1.7 μm) with 0.2% formic acid aqueous solution and acetonitrile as the mobile phase under gradient conditions. Based on the accurate mass measurement (<5 ppm), MS/MS fragmentation patterns and different chromatographic behaviors, a total of 64 compounds were unambiguously or tentatively characterized, including 30 flavonoids, 20 phenolic acids, 12 terpenoids and two phenylpropanoids. Finally, target isolation of three compounds named Acacetin, Rosmarinic acid and Clemastanin A (first isolated from Menthae Haplocalycis herba) were performed based on the obtained results, which further confirmed the deduction of fragmentation patterns and identified the compounds profile in Menthae Haplocalycis herba. Our research firstly systematically elucidated the non-volatile components of Menthae Haplocalycis herba, which laid the foundation for further pharmacological and metabolic studies. Meanwhile, our established method was useful and efficient to screen and identify targeted constituents from traditional Chinese medicine extracts.
Keywords: Menthae Haplocalycis herba; UHPLC-ESI-MS/MS; non-volatile constituents; target isolation Menthae Haplocalycis herba; UHPLC-ESI-MS/MS; non-volatile constituents; target isolation

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MDPI and ACS Style

Xu, L.-L.; Xu, J.-J.; Zhong, K.-R.; Shang, Z.-P.; Wang, F.; Wang, R.-F.; Zhang, L.; Zhang, J.-Y.; Liu, B. Analysis of Non-Volatile Chemical Constituents of Menthae Haplocalycis Herba by Ultra-High Performance Liquid Chromatography-High Resolution Mass Spectrometry. Molecules 2017, 22, 1756. https://doi.org/10.3390/molecules22101756

AMA Style

Xu L-L, Xu J-J, Zhong K-R, Shang Z-P, Wang F, Wang R-F, Zhang L, Zhang J-Y, Liu B. Analysis of Non-Volatile Chemical Constituents of Menthae Haplocalycis Herba by Ultra-High Performance Liquid Chromatography-High Resolution Mass Spectrometry. Molecules. 2017; 22(10):1756. https://doi.org/10.3390/molecules22101756

Chicago/Turabian Style

Xu, Lu-Lu, Jing-Jing Xu, Kun-Rui Zhong, Zhan-Peng Shang, Fei Wang, Ru-Feng Wang, Le Zhang, Jia-Yu Zhang, and Bin Liu. 2017. "Analysis of Non-Volatile Chemical Constituents of Menthae Haplocalycis Herba by Ultra-High Performance Liquid Chromatography-High Resolution Mass Spectrometry" Molecules 22, no. 10: 1756. https://doi.org/10.3390/molecules22101756

APA Style

Xu, L.-L., Xu, J.-J., Zhong, K.-R., Shang, Z.-P., Wang, F., Wang, R.-F., Zhang, L., Zhang, J.-Y., & Liu, B. (2017). Analysis of Non-Volatile Chemical Constituents of Menthae Haplocalycis Herba by Ultra-High Performance Liquid Chromatography-High Resolution Mass Spectrometry. Molecules, 22(10), 1756. https://doi.org/10.3390/molecules22101756

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