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Molecules 2016, 21(5), 549;

Neuroprotective and Cytotoxic Phthalides from Angelicae Sinensis Radix

Modern Research Center for Traditional Chinese Medicine, Shanxi University, No.92, Wucheng Road, Taiyuan 030006, China
Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China
Authors to whom correspondence should be addressed.
Academic Editor: Nancy D. Turner
Received: 8 March 2016 / Revised: 17 April 2016 / Accepted: 21 April 2016 / Published: 26 April 2016
(This article belongs to the Collection Bioactive Compounds)
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Seven phthalides, including a new dimeric one named tokinolide C (7), were isolated from Angelicae Sinensis Radix and characterized. The structures of these compounds were elucidated on the basis of comprehensive analysis of spectroscopic data and comparison with literature data. All of the compounds were evaluated for their cytotoxic activities against the A549, HCT-8, and HepG2 cancer cell lines. Riligustilide (4) showed cytotoxicity against three cancer cell lines, with IC50 values of 13.82, 6.79, and 7.92 μM, respectively. Tokinolide A (6) and tokinolide C (6) exerted low cytotoxicity in these cancer cell lines, while the remaining compounds were inactive. Flow cytometry analysis was employed to evaluate the possible mechanism of cytotoxic action of riligustilide (4). We observed that compound 4 was able to arrest the cell cycle in the G1, S phases and induce apoptosis in a time-dependent manner in HCT-8 cell lines. In addition, these compounds were evaluated for neuroprotective effect against SH-SY5Y cells injured by glutamate. The result showed that ligustilide (1), Z-butylidenephthalide (3) and tokinolide A (6) exhibited significant neuroprotective effects. View Full-Text
Keywords: phthalides; Angelicae Sinensis Radix; cytotoxicity activity; flow cytometry; neuroprotective effect phthalides; Angelicae Sinensis Radix; cytotoxicity activity; flow cytometry; neuroprotective effect

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Gong, W.; Zhou, Y.; Li, X.; Gao, X.; Tian, J.; Qin, X.; Du, G. Neuroprotective and Cytotoxic Phthalides from Angelicae Sinensis Radix. Molecules 2016, 21, 549.

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