Transcriptome Profile Reveals that Pu-Erh Tea Represses the Expression of Vitellogenin Family to Reduce Fat Accumulation in Caenorhabditis elegans

State Key Laboratory of Genetic Resources and Evolutionary & Functional Genomics, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China

Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China

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Pharmaceutical College, Yunnan University of Traditional Chinese Medicine, Kunming 650500, China

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Department of Life Science and Biotechnology, Key Laboratory of Special Biological Resource Development and Utilization of University in Yunnan Province, Kunming University, Kunming 650214, China

Authors to whom correspondence should be addressed.

Academic Editor: Derek J. McPhee

Molecules 2016, 21(10), 1379; https://doi.org/10.3390/molecules21101379

Received: 30 August 2016 / Revised: 4 October 2016 / Accepted: 12 October 2016 / Published: 17 October 2016

(This article belongs to the Special Issue Natural Products in Anti-Obesity Therapy)

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Abstract

Due to misbalanced energy surplus and expenditure, obesity has become a common chronic disorder that is highly associated with many metabolic diseases. Pu-erh tea, a traditional Chinese beverage, has been believed to have numerous health benefits, such as anti-obesity. However, the underlying mechanisms of its anti-obesity effect are yet to be understood. Here, we take the advantages of transcriptional profile by RNA sequencing (RNA-Seq) to view the global gene expression of Pu-erh tea. The model organism Caenorhabditis elegans was treated with different concentrations of Pu-erh tea water extract (PTE, 0 g/mL, 0.025 g/mL, and 0.05 g/mL). Compared with the control, PTE indeed decreases lipid droplets size and fat accumulation. The high-throughput RNA-Sequence technique detected 18073 and 18105 genes expressed in 0.025 g/mL and 0.05 g/mL PTE treated groups, respectively. Interestingly, the expression of the vitellogenin family (vit-1, vit-2, vit-3, vit-4 and vit-5) was significantly decreased by PTE, which was validated by qPCR analysis. Furthermore, vit-1(ok2616), vit-3(ok2348) and vit-5(ok3239) mutants are insensitive to PTE triggered fat reduction. In conclusion, our transcriptional profile by RNA-Sequence suggests that Pu-erh tea lowers the fat accumulation primarily through repression of the expression of vit(vitellogenin) family, in addition to our previously reported (sterol regulatory element binding protein) SREBP-SCD (stearoyl-CoA desaturase) axis. View Full-Text

Keywords: Pu-erh tea water extract (PTE); fat accumulation; RNA-Seq; vitellogenin (vit) family

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MDPI and ACS Style

Xiao, R.-Y.; Hao, J.; Ding, Y.-H.; Che, Y.-Y.; Zou, X.-J.; Liang, B. Transcriptome Profile Reveals that Pu-Erh Tea Represses the Expression of Vitellogenin Family to Reduce Fat Accumulation in Caenorhabditis elegans. Molecules 2016, 21, 1379. https://doi.org/10.3390/molecules21101379

AMA Style

Xiao R-Y, Hao J, Ding Y-H, Che Y-Y, Zou X-J, Liang B. Transcriptome Profile Reveals that Pu-Erh Tea Represses the Expression of Vitellogenin Family to Reduce Fat Accumulation in Caenorhabditis elegans. Molecules. 2016; 21(10):1379. https://doi.org/10.3390/molecules21101379

Chicago/Turabian Style

Xiao, Ru-Yue, Junjun Hao, Yi-Hong Ding, Yan-Yun Che, Xiao-Ju Zou, and Bin Liang. 2016. "Transcriptome Profile Reveals that Pu-Erh Tea Represses the Expression of Vitellogenin Family to Reduce Fat Accumulation in Caenorhabditis elegans" Molecules 21, no. 10: 1379. https://doi.org/10.3390/molecules21101379

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Transcriptome Profile Reveals that Pu-Erh Tea Represses the Expression of Vitellogenin Family to Reduce Fat Accumulation in Caenorhabditis elegans

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