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Volume 20
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10.3390/molecules201018717
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Article

Enzymatic Hydrolysis and Simultaneous Extraction for Preparation of Genipin from Bark of Eucommia ulmoides after Ultrasound, Microwave Pretreatment

by
Lili Li
1,2,
Yupin Guo
3,
Lianfei Zhao
1,2,
Yuangang Zu
1,2,
Huiyan Gu
4,* and
Lei Yang
1,2,*
1
Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China
2
State Engineering Laboratory for Bioresource Eco-Utilization, Northeast Forestry University, Harbin 150040, China
3
College of Animal Science and Technology, Hebei North University, Zhangjiakou 075000, China
4
School of Forestry, Northeast Forestry University, Harbin 150040, China
*
Authors to whom correspondence should be addressed.
Molecules 2015, 20(10), 18717-18731; https://doi.org/10.3390/molecules201018717
Submission received: 10 July 2015 / Revised: 22 September 2015 / Accepted: 12 October 2015 / Published: 15 October 2015
(This article belongs to the Section Natural Products Chemistry)
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Abstract

A continuous process based on the combination of ultrasounds and/or microwaves pretreatments followed by enzymatic hydrolysis and simultaneous extraction (EHSE) has been proposed to recover genipin from Eucommia ulmoides bark. At first, in the pretreatment step, the mixture of 1.0 g dried bark powder and 10 mL deionized water were irradiated by microwave under 500 W for 10 min. Then, in hydrolysis step, the optimal conditions were as follows: 0.5 mg/mL of cellulase concentration, 4.0 pH of enzyme solution, 24 h of incubation time and 40 °C of incubation temperature. After incubation, 10 mL ethanol was added to extract genipin for 30 min by ultrasound. After EHSE treatment, the yield of genipin could reach 1.71 μmol/g. Moreover, scanning electron micrographs illustrated that severe structural disruption of plant was obtained by EHSE. The results indicated that the EHSE method provided a good alternative for the preparation of genipin from Eucommia ulmoides bark as well as other herbs.
Keywords: enzymatic hydrolysis and simultaneous extraction; Eucommia ulmoides bark; genipin; ultrasound/microwave irradiation pretreatment enzymatic hydrolysis and simultaneous extraction; Eucommia ulmoides bark; genipin; ultrasound/microwave irradiation pretreatment

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MDPI and ACS Style

Li, L.; Guo, Y.; Zhao, L.; Zu, Y.; Gu, H.; Yang, L. Enzymatic Hydrolysis and Simultaneous Extraction for Preparation of Genipin from Bark of Eucommia ulmoides after Ultrasound, Microwave Pretreatment. Molecules 2015, 20, 18717-18731. https://doi.org/10.3390/molecules201018717

AMA Style

Li L, Guo Y, Zhao L, Zu Y, Gu H, Yang L. Enzymatic Hydrolysis and Simultaneous Extraction for Preparation of Genipin from Bark of Eucommia ulmoides after Ultrasound, Microwave Pretreatment. Molecules. 2015; 20(10):18717-18731. https://doi.org/10.3390/molecules201018717

Chicago/Turabian Style

Li, Lili, Yupin Guo, Lianfei Zhao, Yuangang Zu, Huiyan Gu, and Lei Yang. 2015. "Enzymatic Hydrolysis and Simultaneous Extraction for Preparation of Genipin from Bark of Eucommia ulmoides after Ultrasound, Microwave Pretreatment" Molecules 20, no. 10: 18717-18731. https://doi.org/10.3390/molecules201018717

APA Style

Li, L., Guo, Y., Zhao, L., Zu, Y., Gu, H., & Yang, L. (2015). Enzymatic Hydrolysis and Simultaneous Extraction for Preparation of Genipin from Bark of Eucommia ulmoides after Ultrasound, Microwave Pretreatment. Molecules, 20(10), 18717-18731. https://doi.org/10.3390/molecules201018717

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