CopA3 Peptide Prevents Ultraviolet-Induced Inhibition of Type-I Procollagen and Induction of Matrix Metalloproteinase-1 in Human Skin Fibroblasts
Abstract
:1. Introduction
2. Results and Discussion
2.1. CCD-986sk Fibroblast Cell Viability Measured by MTT Assay
2.2. Effect of CopA3 on Type-I Procollagen Synthesis and Matrix Metalloproteinase-1 Levels
2.3. mRNA of MMP-1 was Decreased in CCD-986sk Cells by CopA3
2.4. UVB Induced Aging Related MMP-1 Protein Expression was Suppressed by CopA3
3. Experimental
3.1. CopA3 (Disulfide Dimer) Synthesis, Dimer Peptide Structure Determination
3.2. Cell Culture
3.3. Cell Viability
3.4. Determination of MMP-1 and Type-I Procollagen
3.5. cDNA Synthesis and RT-PCR
Gene | Primer | Sequence (5' → 3') |
---|---|---|
MMP-1 | Forward | AGC GTG TGA CAG TAA GCT AA |
Reverse | GTT TTC CTC AGA AAG AGC AGC AT | |
β-actin | Forward | ATT GTT GCC ATC AAT GAC CC |
Reverse | AGT AGA GGC AGG GAT GAT |
3.6. Western Blotting
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Kim, D.-H.; Kim, H.-H.; Kim, H.-J.; Jung, H.-G.; Yu, J.-M.; Lee, E.-S.; Cho, Y.-H.; Kim, D.-I.; An, B.-J. CopA3 Peptide Prevents Ultraviolet-Induced Inhibition of Type-I Procollagen and Induction of Matrix Metalloproteinase-1 in Human Skin Fibroblasts. Molecules 2014, 19, 6407-6414. https://doi.org/10.3390/molecules19056407
Kim D-H, Kim H-H, Kim H-J, Jung H-G, Yu J-M, Lee E-S, Cho Y-H, Kim D-I, An B-J. CopA3 Peptide Prevents Ultraviolet-Induced Inhibition of Type-I Procollagen and Induction of Matrix Metalloproteinase-1 in Human Skin Fibroblasts. Molecules. 2014; 19(5):6407-6414. https://doi.org/10.3390/molecules19056407
Chicago/Turabian StyleKim, Dong-Hee, Han-Hyuk Kim, Hyeon-Jeong Kim, Hyun-Gug Jung, Jae-Myo Yu, Eun-Su Lee, Yong-Hun Cho, Dong-In Kim, and Bong-Jeun An. 2014. "CopA3 Peptide Prevents Ultraviolet-Induced Inhibition of Type-I Procollagen and Induction of Matrix Metalloproteinase-1 in Human Skin Fibroblasts" Molecules 19, no. 5: 6407-6414. https://doi.org/10.3390/molecules19056407