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Open AccessArticle

Anti-Proliferative Effects of Siegesbeckia orientalis Ethanol Extract on Human Endometrial RL-95 Cancer Cells

1
Institute of Biotechnology and Chemical Engineering, I-Shou University, Kaohsiung 84001, Taiwan
2
Department of Obstetrics & Gynecology, E-Da Hospital, E-Da Hospital/I-Shou University, Kaohsiung 82445, Taiwan
3
Department of Nutrition, I-Shou University, Kaohsiung 82445, Taiwan
4
Department of Biomedical Engineering, I-Shou University, Kaohsiung 82445, Taiwan
*
Author to whom correspondence should be addressed.
Molecules 2014, 19(12), 19980-19994; https://doi.org/10.3390/molecules191219980
Received: 13 September 2014 / Revised: 18 November 2014 / Accepted: 21 November 2014 / Published: 1 December 2014
(This article belongs to the Collection Bioactive Compounds)
Endometrial cancer is a common malignancy of the female genital tract. This study demonstrates that Siegesbeckia orientalis ethanol extract (SOE) significantly inhibited the proliferation of RL95-2 human endometrial cancer cells. Treating RL95-2 cells with SOE caused cell arrest in the G2/M phase and induced apoptosis of RL95-2 cells by up-regulating Bad, Bak and Bax protein expression and down-regulation of Bcl-2 and Bcl-xL protein expression. Treatment with SOE increased protein expression of caspase-3, -8 and -9 dose-dependently, indicating that apoptosis was through the intrinsic and extrinsic apoptotic pathways. Moreover, SOE was also effective against A549 (lung cancer), Hep G2 (hepatoma), FaDu (pharynx squamous cancer), MDA-MB-231 (breast cancer), and especially on LNCaP (prostate cancer) cell lines. In total, 10 constituents of SOE were identified by Gas chromatography-mass analysis. Caryophyllene oxide and caryophyllene are largely responsible for most cytotoxic activity of SOE against RL95-2 cells. Overall, this study suggests that SOE is a promising anticancer agent for treating endometrial cancer. View Full-Text
Keywords: Siegesbeckia orientalis; RL95-2 endometrial cancer cells; cytotoxicity; apoptosis; ingredient analysis Siegesbeckia orientalis; RL95-2 endometrial cancer cells; cytotoxicity; apoptosis; ingredient analysis
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Chang, C.-C.; Hsu, H.-F.; Huang, K.-H.; Wu, J.-M.; Kuo, S.-M.; Ling, X.-H.; Houng, J.-Y. Anti-Proliferative Effects of Siegesbeckia orientalis Ethanol Extract on Human Endometrial RL-95 Cancer Cells. Molecules 2014, 19, 19980-19994.

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